Journal ArticleDOI
Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.)
TLDR
The ploidy of 12 randomly selected regenerants was assessed by chromosome counts in root tips and showed no segregation for morphological traits in the progeny, suggesting that they were spontaneously doubled haploids.Abstract:
Morphogenic calli were obtained efficiently from ab initio cultures of isolated microspores in eggplant. Initial culture of freshly isolated microspores in sucrose-free medium at high temperature (35°C) for 3 d was a prerequisite for callus induction. The microspores were re-cultured in modified NLN medium containing 2% sucrose and phytohormones (NAA 0.5 mg l(-1), BA 0.5 mg l(-1)) in the dark. After 4 weeks of re-culture, small calli derived from microspores were transferred to MS medium containing 4 mg l(-1) zeatin and 0.2 mg l(-1) IAA for shoot regeneration. The ploidy of 12 randomly selected regenerants was assessed by chromosome counts in root tips. Only one of the regenerants was haploid, 7 were diploid, 3 were triploid and one was tetraploid. The diploids set seeds after self-pollination and showed no segregation for morphological traits in the progeny, suggesting that they were spontaneously doubled haploids.read more
Citations
More filters
Journal ArticleDOI
Stresses applied for the re‐programming of plant microspores towards in vitro embryogenesis
TL;DR: An overview of various stresses and mechanisms of action of these stresses in inducing microspore embryogenesis is given and whether these stresses are effective in a wider range of species is seen.
Journal ArticleDOI
Common and Distinct Functions of Arabidopsis Class A1 and A2 Heat Shock Factors in Diverse Abiotic Stress Responses and Development
Hsiang-chin Liu,Yee-yung Charng +1 more
TL;DR: In this article, a Cauliflower mosaic virus 35S promoter was used to induce the heat shock factor (HSF) homologs in Arabidopsis (Arabidopsis thaliana) to activate the transcription of heat-induced genes, including HSFA2.
Journal ArticleDOI
Applications of biotechnology in eggplant
Cécile Collonnier,Isabelle Fock,V. Kashyap,Giuseppe Leonardo Rotino,Marie-Christine Daunay,Y. Lian,Ika Mariska,Manchikatla Venkat Rajam,Aline Servaes,G. Ducreux,Darasinh Sihachakr +10 more
TL;DR: Only resistance against insects, and parthenocarpic fruit development have successfully been developed in eggplant using Agrobacterium tumefasciens transformation, however, some work on genetic engineering of eggplant for other biotic and abiotic stresses has recently been initiated.
Journal ArticleDOI
Biotechnology of eggplant
Vikas Kashyap,S. Vinod Kumar,Cécile Collonnier,F Fusari,Robert Haïcour,Giuseppe Leonardo Rotino,Darasinh Sihachakr,Manchikatla Venkat Rajam +7 more
TL;DR: Eggplant genetics is summarized with an emphasis on the use of biotechnology to introgress genes from wild species into cultivated eggplant for improvement of other agronomic traits, including disease and pest resistance, and quality and shelf life of fruits.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids
TL;DR: Stable integration of fulllength microinjected genes into high molecular weight DNA was proven by Southern analysis of genomic DNA isolated from regenerated plants.
Journal ArticleDOI
The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts.
TL;DR: Selfed seed from the M-37 plants have provided tolerance to CS in both greenhouse and field tests, and the microspore selection system highlighted as a new method for in vitro selection.
Journal ArticleDOI
High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L.
TL;DR: Conditions for reliable induction of embryogenesis from isolated microspores of Brassica oleracea var.
Journal ArticleDOI
Development of microspores in vivo and in vitro in Brassica napus L.
TL;DR: Populations of highly homogeneous uninucleate and binucleate microspores of Brassica napus cv.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more