Journal ArticleDOI
Carbon and nitrogen sources modulate lipase production in the yeast Yarrowia lipolytica
Patrick Fickers,Jean-Marc Nicaud,Claude Gaillardin,Jacqueline Destain,Philippe Thonart,Philippe Thonart +5 more
TLDR
To analyse the influence of nitrogen and carbon sources on extracellular lipase production by Yarrowia lipolytica‐overproducing mutant in order to optimize its production in large‐scale bioreactors.Abstract:
Aims: To analyse the influence of nitrogen and carbon sources on extracellular lipase production by Yarrowia lipolytica-overproducing mutant in order to optimize its production in large-scale bioreactors.
Methods and Results: The level of lipase production and LIP2 induction, measured using an LIP2–LacZ reporter gene, were compared for different carbon and nitrogen sources and for different concentrations. The localization of the enzyme during growth was also determined by Western blotting analysis using a six-histidine-tagged lipase.
Significance and Impact of the Study: Tryptone N1 and oleic acid are the most suitable nitrogen and carbon sources for the production of the extracellular lipase by the Y. lipolytica mutant. Higher levels of lipase production were obtained as the tryptone concentration increased in the culture medium. Such a positive correlation was not observed with oleic acid media where the highest lipolytic productivities were obtained in the presence of low concentration. We also demonstrate that in the presence of oleic acid, lipase is cell-bound during the growth phase before being released in the media.
Conclusions: This work provides a better understanding of the mechanism controlling LIP2 expression and, thus, extracellular lipase production in the yeast Y. lipolytica.read more
Citations
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Journal ArticleDOI
Production of recombinant proteins by yeast cells
Eda Çelik,Pınar Çalık +1 more
TL;DR: The present status of the main and most promising yeast expression systems is discussed and it is shown that with a large variety of vectors, promoters and selection markers to choose from, it is possible to design more cost-effective expression systems in order to meet the increasing demand for recombinant proteins and glycoproteins.
Journal ArticleDOI
The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production.
TL;DR: A preliminary cost analysis shows that VFAs-based biodiesel production is competitive with current palm and soybean based biodiesels and further process development for lower aeration cost and higher lipid yield will make this process more economical.
Journal ArticleDOI
The lipases from Yarrowia lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications.
TL;DR: Crystallographic data highlight that the Lip2p lipase is a lipase sensu stricto with a lid covering the active site of the enzyme in its closed conformation, confirming the high adaptation of Y. lipolytica to hydrophobic substrate utilization.
Journal ArticleDOI
Influence of Glucose and Saturated Free-Fatty Acid Mixtures on Citric Acid and Lipid Production by Yarrowia lipolytica
Seraphim Papanikolaou,Maria Galiotou-Panayotou,Isabelle Chevalot,Michael Komaitis,Ivan Marc,George Aggelis +5 more
TL;DR: Numerical models that were used in order to quantify the kinetic behavior of the above Yarrowia lipolytica strain showed successful simulation, while the optimized parameter values were similar to those experimentally measured and the predictive ability of the models was satisfactory.
Journal ArticleDOI
Yarrowia lipolytica and Its Multiple Applications in the Biotechnological Industry
TL;DR: This review discusses the biotechnological potential of Y. lipolytica and the best growing conditions for production of some metabolites of bioteschnological interest, and the choice of carbon and nitrogen sources to be used.
References
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Action de la lipase pancréatique sur les esters en émulsion
L. Sarda,P. Desnuelle +1 more
TL;DR: De considerer la lipase pancreatique comme une esterase particuliere destinee a agir sur des esters insolubles au niveau of l'interface separant ces esters de l'eau, un calcul fait intervenir the “concentration molaire interfaciale” du substrat.
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