Journal ArticleDOI
Cell growth and division in hypertonic medium
TLDR
Cells exposed to NaCl-supplemented medium exhibit a concentration dependent inhibition of macromolecular synthesis, protein synthesis being most affected, DNA synthesis less so and RNA synthesis least, except at slightly elevated tonicities which often produced a slight stimulation of the rate of synthetic activity.About:
This article is published in Experimental Cell Research.The article was published on 1974-08-01. It has received 34 citations till now. The article focuses on the topics: Tonicity & Metaphase.read more
Citations
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Journal ArticleDOI
Functional Significance of Cell Volume Regulatory Mechanisms
Florian Lang,Gillian L. Busch,Markus Ritter,Harald Völkl,Siegfried Waldegger,Erich Gulbins,Dieter Häussinger +6 more
TL;DR: Cell volume may be considered a second message in the transmission of hormonal signals, and alterations of cell volume and volume regulatory mechanisms participate in a wide variety of cellular functions including epithelial transport, metabolism, excitation, hormone release, migration, cell proliferation, and cell death.
Journal ArticleDOI
p38 Kinase activity is essential for osmotic induction of mRNAs for HSP70 and transporter for organic solute betaine in Madin-Darby canine kidney cells
David Sheikh-Hamad,John F. Di Mari,Wadi N. Suki,Robert L. Safirstein,Bruns A. Watts,D. Rouse +5 more
TL;DR: It is shown that inhibition of p38 kinase activity, using the specific inhibitor SB203580, abolishes the hypertonicity-mediated induction of mRNAs for HSP70 and betaine transporter in Madin-Darby canine kidney cells and that the increased activity of JNK-1 during p38Kinase inhibition is consistent with regulation of J NK-1 by p38 Kinase in osmotically stressed cells.
Journal ArticleDOI
Alkaline phosphatase in HT-29, a human colon cancer cell line: influence of sodium butyrate and hyperosmolality.
TL;DR: HT-29, a cell line derived from a human colon carcinoma, exhibits very low alkaline phosphatase activity, and is of the intestinal type, while 5-Bromo-2′-deoxyuridine and inhibitors of DNA synthesis cause a slight increase in specific activity.
Journal Article
The stimulation by methotrexate of human chorionic gonadotropin and placental alkaline phosphatase in cultured choriocarcinoma cells.
TL;DR: Treatment of the BeWo line of choriocarcinoma cells with methotrexate in doses that inhibit DNA synthesis causes a tenfold increase in synthesis of human chorionic gonadotropin and a threefold increased in activity of placental alkaline phosphatase.
References
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Journal ArticleDOI
The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system.
John M. Kissane,Eli Robins +1 more
Journal ArticleDOI
Determination of nucleic acids in animal tissues
TL;DR: A modification of the Barrenscheen and Peham method for ribose determination with orcinol was worked out in order to determine pentose nucleic acid (PNA) in the same organ extracts, which permitted the evaluation of DNA in amounts as small as 2.5 y per ml.
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Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells.
T. Terasima,L.J. Tolmach +1 more
TL;DR: Synchronization of HeLa S3 cells may be effected by a purely selective method which takes advantage of the tenuous binding of cells to the growth surface during mitosis, and synchronization of the population appears to arise largely during the G 1 phase.
Journal Article
Studies on unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency.
TL;DR: Thymidine-deficient growth of HeLa cells induced by either of two agents, amethopterin or 5-fluorouracil-2′-deoxyriboside, resulted in an unbalanced growth syndrome strikingly similar to that described for bacteria.
Journal ArticleDOI
Comparison of mitotic phenomena and effects induced by hypertonic solutions in HeLa cells.
TL;DR: The prophase-like condensation of the chromatin following exposure of the intact cell to hypertonic medium cannot be reproduced on an ultrastructural level in the isolated nucleus with any known variation in salt concentration, suggesting significant modifications of the nuclear contents during isolation.