BookDOI
Cell Viability Assays
Daniel Gilbert,Oliver Friedrich +1 more
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TLDR
This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures using the formation of the omnipresent reducing agents NADH and NADPH as a marker for metabolic activity in the following assays.Abstract:
This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell’s metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in the following assays. Using NADH and NADPH as electron sources, specific dyes are biochemically reduced which results in a color change that can be determined with basic photometrical methods. The assays selected for this chapter include MTT, WST, and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.read more
Citations
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Toxicity Assessment of Nanomaterials
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Effects of Cryoprotective Medium Composition, Dilution Ratio, and Freezing Rates on Spotted Halibut (Verasper variegatus) Sperm Cryopreservation.
TL;DR: The effect of several factors for successful cryopreservation of fish sperm including cryoprotective agents (CPAs), diluents, dilution ratios, and freezing rates are investigated to develop an optimal sperm cryop Reservation protocol for spotted halibut.
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Cellular Antioxidant Activity of Olive Pomace Extracts: Impact of Gastrointestinal Digestion and Cyclodextrin Encapsulation.
TL;DR: Investigation of the protective effect of a polyphenolic extract from olive pomace on cell viability and antioxidant defense of cultured human HepG2 cells submitted to oxidative stress induced by tert-butylhydroperoxide shows that OPE is more potent antioxidant in comparison to equivalent dose of main polyphenols (HTS and TS).
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Quality screening of the Lagos lagoon sediment by assessing the cytotoxicity and toxicological responses of rat hepatoma H4IIE and fish PLHC-1 cell-lines using different extraction approaches.
TL;DR: Although the different extracts did not exert high cytotoxic effects (except the non-polar) at the tested concentrations, they significantly modulated phase I biotransformation responses, showing that the studied sediments contain complex chemical mixture in the different Extracts, with potential for overt physiological and general health consequences.
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Trichinella spiralis Calreticulin S-Domain Binds to Human Complement C1q to Interfere With C1q-Mediated Immune Functions.
Shuai Shao,Chunyue Hao,Bin Zhan,Qinghui Zhuang,Limei Zhao,Yi Chen,Jingjing Huang,Xinping Zhu +7 more
TL;DR: Findings provide evidence that theTsCRT-S fragment, rather than the full-length TsCRT, is a potential target for vaccine or therapeutic development for trichinellosis, as well as for complement-related autoimmune disease therapies.
References
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Journal ArticleDOI
Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
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Synchronization of Plasmodium falciparum erythrocytic stages in culture.
TL;DR: Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture.
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Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique.
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Neutral red uptake assay for the estimation of cell viability/cytotoxicity.
TL;DR: The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture and is cheaper and more sensitive than other cytotoxicity tests (tetrazolium salts, enzyme leakage or protein content).
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Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening
TL;DR: A side-by-side comparison of this new fluorescence assay and a standard radioisotopic method suggest that it may be an ideal method for high-throughput antimalarial drug screening.
Related Papers (5)
Cell Viability Assays -- Assay Guidance Manual
Sittampalam Gs,Grossman A,Brimacombe K,Arkin M,Auld D,Austin C,Baell J,Bejcek B,Caaveiro Jmm,Chung Tdy,Nathan P. Coussens,Dahlin Jl,Devanaryan,Foley Tl,Glicksman M,Hall,Haas Jv,Hoare Srj,Inglese J,Iversen Pw,Kahl Sd,Stephen C. Kales,Kirshner S,Lal-Nag M,Li Z,McGee J,McManus O,Riss T,Saradjian P,Trask Oj,Weidner,Wildey Mj,Xia M,Xu X +33 more