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Cell Viability Assays
Daniel Gilbert,Oliver Friedrich +1 more
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This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures using the formation of the omnipresent reducing agents NADH and NADPH as a marker for metabolic activity in the following assays.Abstract:
This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell’s metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in the following assays. Using NADH and NADPH as electron sources, specific dyes are biochemically reduced which results in a color change that can be determined with basic photometrical methods. The assays selected for this chapter include MTT, WST, and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.read more
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Chitosan and carboxymethyl cellulose-based 3D multifunctional bioactive hydrogels loaded with nano-curcumin for synergistic diabetic wound repair.
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TL;DR: In this article , the Chitosan-CMC-g-PF127 injectable hydrogels are developed using solvent casting and the results revealed promising tissue regenerative ability and showed enhancement of fibroblasts, keratinocytes, and collagen deposition, which stimulates the epidermal junction.
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Engineered contrast agents in a single structure for T1-T2 dual magnetic resonance imaging.
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Mechanism of CK2.3, a Novel Mimetic Peptide of Bone Morphogenetic Protein Receptor Type IA, Mediated Osteogenesis.
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Withanolide C Inhibits Proliferation of Breast Cancer Cells via Oxidative Stress-Mediated Apoptosis and DNA Damage
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α-Glucosidase Inhibitory and Glucose Uptake Stimulatory Effects of Phenolic Compounds From Dendrobium christyanum:
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TL;DR: A methanolic extract from the dried root of Dendrobium christyanum Rchb.f. (Orchidaceae) exhibited α-glucosidase inhibitory activity and glucose uptake stimulatory effect as mentioned in this paper.
References
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Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
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Cell Viability Assays -- Assay Guidance Manual
Sittampalam Gs,Grossman A,Brimacombe K,Arkin M,Auld D,Austin C,Baell J,Bejcek B,Caaveiro Jmm,Chung Tdy,Nathan P. Coussens,Dahlin Jl,Devanaryan,Foley Tl,Glicksman M,Hall,Haas Jv,Hoare Srj,Inglese J,Iversen Pw,Kahl Sd,Stephen C. Kales,Kirshner S,Lal-Nag M,Li Z,McGee J,McManus O,Riss T,Saradjian P,Trask Oj,Weidner,Wildey Mj,Xia M,Xu X +33 more