BookDOI
Cell Viability Assays
Daniel Gilbert,Oliver Friedrich +1 more
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TLDR
This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures using the formation of the omnipresent reducing agents NADH and NADPH as a marker for metabolic activity in the following assays.Abstract:
This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell’s metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in the following assays. Using NADH and NADPH as electron sources, specific dyes are biochemically reduced which results in a color change that can be determined with basic photometrical methods. The assays selected for this chapter include MTT, WST, and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.read more
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Comparison of Effects of Curcumin and Nano-curcumin on the Survival of Human-Derived Mesenchymal Stem Cells: An Experimental Study:
Pinjari Hameeda,Sandeep Katti,Rajkishore Jammalamadugu,Kishore Bhatt,Malleswara Rao Peram,Vijay Kumbar +5 more
TL;DR: Both CUR and N-CUR have a dose-dependent effect on human derived MSCs survival when incubated for 48 hr, whereas N- CUR shows increased cell survival rate even at 72’hr of incubation.
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A systems toxicology framework for improving the identification of paracetamol overdose
TL;DR: A systems toxicology approach is taken in an attempt to provide further insight into the APAP overdose problem, and propose potential improvements to the current treatment framework.
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The Effect of Aflatoxin B1 on Tumor-Related Genes and Phenotypic Characters of MCF7 and MCF10A Cells
Mowaffaq Adam Ahmed Adam,Laina Zarisa Mohd Kamal,Mahibub Mahamadsa Kanakal,Dinesh Babu,Saad S. Dahham,Yasser M Tabana,Bronwyn Lok,Brittany M. Bermoy,Muhammad Yunus,Leslie Thian Lung Than,Khaled Barakat,Doblin Sandai +11 more
TL;DR: The effects of AB1 on the phenotype and epigenetics of both MCF7 and MCF10A cells associated with cancer development observed in this study suggest that AB1 is a potential risk factor for developing breast cancer.
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Enhancement of Inhibition of the Pseudomonas sp. Biofilm Formation on Bacterial Cellulose-Based Wound Dressing by the Combined Action of Alginate Lyase and Gentamicin
Magdalena Charęza,Katarzyna Przygrodzka,Anna Żywicka,Bartłomiej Grygorcewicz,Peter Sobolewski,Sylwia Mozia,Marcin Smiglak,Radosław Drozd +7 more
TL;DR: In this article , the promising therapeutic properties of alginate lyase (AlgL) immobilised on BC membranes for protecting wounds from Pseudomonas aeruginosa infection were investigated.
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Chemical characterization and antineoplastic effect of oligosaccharides from Cabernet Franc red wine in mammary tumor model in mice.
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TL;DR: In this article , the authors characterized oligosaccharide compounds (Oligo) in Cabernet Franc red wine and investigated its antineoplastic effects against mammary tumor cells in vivo and in vitro.
References
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Journal ArticleDOI
Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
Journal ArticleDOI
Synchronization of Plasmodium falciparum erythrocytic stages in culture.
TL;DR: Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture.
Journal ArticleDOI
Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique.
TL;DR: A rapid, semiautomated microdilution method was developed for measuring the activity of potential antimalarial drugs against cultured intraerythrocytic asexual forms of the human malaria parasite Plasmodium falciparum, and results demonstrated that the method is sensitive and precise.
Journal ArticleDOI
Neutral red uptake assay for the estimation of cell viability/cytotoxicity.
TL;DR: The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture and is cheaper and more sensitive than other cytotoxicity tests (tetrazolium salts, enzyme leakage or protein content).
Journal ArticleDOI
Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening
TL;DR: A side-by-side comparison of this new fluorescence assay and a standard radioisotopic method suggest that it may be an ideal method for high-throughput antimalarial drug screening.
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Cell Viability Assays -- Assay Guidance Manual
Sittampalam Gs,Grossman A,Brimacombe K,Arkin M,Auld D,Austin C,Baell J,Bejcek B,Caaveiro Jmm,Chung Tdy,Nathan P. Coussens,Dahlin Jl,Devanaryan,Foley Tl,Glicksman M,Hall,Haas Jv,Hoare Srj,Inglese J,Iversen Pw,Kahl Sd,Stephen C. Kales,Kirshner S,Lal-Nag M,Li Z,McGee J,McManus O,Riss T,Saradjian P,Trask Oj,Weidner,Wildey Mj,Xia M,Xu X +33 more