Journal ArticleDOI
Synchronization of Plasmodium falciparum erythrocytic stages in culture.
TLDR
Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture.Abstract:
Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture. Immediately after sorbitol treatment, cultures consisted mainly of single and multiple ring-form infections. At the same time, varying degrees of lysis of erythrocytes infected with the more mature stages of the parasite was evident. Approximately 95% of the parasites were in the ring stage of development at 48 and 96 hr after sorbitol treatment-likewise, a high percentage of trophozoite and schizont stages was observed at 24, 72, and 120 hr. D-Mannitol produced similar, selective, lytic effects.read more
Citations
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Mutations in the P. falciparum Digestive Vacuole Transmembrane Protein PfCRT and Evidence for Their Role in Chloroquine Resistance
David A. Fidock,Takashi Nomura,Angela K. Talley,Roland A. Cooper,Sergey M. Dzekunov,Michael T. Ferdig,Lyann M. B. Ursos,Amar Bir Singh Sidhu,Bronwen Naudé,Kirk W. Deitsch,Xin-zhuan Su,John C. Wootton,Paul D. Roepe,Thomas E. Wellems +13 more
TL;DR: The determinant of verapamil-reversible chloroquine resistance (CQR) in a Plasmodium falciparum genetic cross maps to a 36 kb segment of chromosome 7 that harbors a 13-exon gene, pfcrt, having point mutations that associate completely with CQR in parasite lines from Asia, Africa, and South America.
Journal ArticleDOI
Discovery of Gene Function by Expression Profiling of the Malaria Parasite Life Cycle
Karine G. Le Roch,Yingyao Zhou,Peter L. Blair,Muni Grainger,J. Kathleen Moch,J. David Haynes,Patricia de la Vega,Anthony A. Holder,Serge Batalov,Daniel J. Carucci,Elizabeth A. Winzeler,Elizabeth A. Winzeler +11 more
TL;DR: A high-density oligonucleotide array is used to generate expression profiles of human and mosquito stages of the malaria parasite's life cycle and finds genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes.
Journal ArticleDOI
Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening
TL;DR: A side-by-side comparison of this new fluorescence assay and a standard radioisotopic method suggest that it may be an ideal method for high-throughput antimalarial drug screening.
Journal ArticleDOI
Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum.
YongKeun Park,Monica Diez-Silva,Gabriel Popescu,George Lykotrafitis,Wonshik Choi,Michael S. Feld,Subra Suresh +6 more
TL;DR: Two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs are investigated and offer potential avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.
BookDOI
Cell Viability Assays
Daniel Gilbert,Oliver Friedrich +1 more
TL;DR: This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures using the formation of the omnipresent reducing agents NADH and NADPH as a marker for metabolic activity in the following assays.
References
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Journal ArticleDOI
Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
Journal ArticleDOI
Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method.
James B. Jensen,William Trager +1 more
TL;DR: Full details of the petri dish method and its application in demonstrating the feasibility of using outdated erythrocytes and for testing certain other modifications of the culture conditions are presented.
Journal ArticleDOI
Cultivation of malarial parasites
William Trager,James B. Jenson +1 more
TL;DR: The method for continuous cultivation of Plasmodium falciparum has now been successfully applied to several strains from different geographical areas and has been used for tests of antimalarial drugs, studies of parasite–host cell interactions with special reference to sickle haemoglobin, and for the production of amounts of parasite material sufficient for experimental immunisation of Aotus trivirgatus monkeys.
Journal ArticleDOI
Cryoprotectants—a new class of drugs
TL;DR: Research in cryobiology offers new insights to the role of water in living systems as well as offering a method of suspended animation.
Journal ArticleDOI
Protein Synthesis in Vitro by Cryopreserved Plasmodium Falciparum
TL;DR: The specificity of protein synthesis as an activity of the parasites was shown by the inactivity of control uninfected erythrocytes stored by the same method, and evidence for the specificity of the reaction was obtained from inhibition studies with chloroquine.
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