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Cell Viability Assays
Daniel Gilbert,Oliver Friedrich +1 more
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This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures using the formation of the omnipresent reducing agents NADH and NADPH as a marker for metabolic activity in the following assays.Abstract:
This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell’s metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in the following assays. Using NADH and NADPH as electron sources, specific dyes are biochemically reduced which results in a color change that can be determined with basic photometrical methods. The assays selected for this chapter include MTT, WST, and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.read more
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Recursive model for dose-time responses in pharmacological studies.
TL;DR: A parametric model for dose-time responses that follows Gompertz law in time and Hill equation across dose approximately is proposed and exhibits a superior performance compared to the individual ones for both synthetic data and actual pharmacological data.
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Polysaccharides from green sweet pepper increase the antineoplastic effect of methotrexate on mammary tumor cells
Eliana Rezende Adami,Claudia Rita Corso,Natalia Mulinari Turin-Oliveira,Claudia Martins Galindo,Letícia Milani,Maria Carolina Stipp,Liziane Cristine Malaquias da Silva,Georgia Erdmann do Nascimento,Pedro Felipe Pereira Chaves,Andressa Chequin,Marihá Mariott,Luisa Mota da Silva,Giseli Klassen,Edneia A.S. Ramos,Lucimara M.C. Cordeiro,Alexandra Acco +15 more
TL;DR: Cap exerted antineoplastic effects in vivo and in vitro against mammary tumor cells, possibly by modulating inflammation and angiogenesis, and may be a promising adjunct chemotherapy with lower toxicity.
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Increasing Cellular Uptake and Permeation of Curcumin Using a Novel Polymer-Surfactant Formulation
TL;DR: In this paper , the authors investigated the potential for novel polymer-surfactant-based formulations (designated A and B) to improve the solubility and permeability of curcumin.
Journal ArticleDOI
Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system.
Sin-Yeang Teow,Sin-Yeang Teow,Kitson Liew,Mohd Firdaus Che Mat,Marini Marzuki,Norazlin Abdul Aziz,Tai-Lin Chu,Munirah Ahmad,Alan Soo-Beng Khoo +8 more
TL;DR: XenoLuc assay is specific, sensitive, rapid and cost-effective for measuring the growth of luciferase-expressing cells in a co- or multiple-culture system and may be adapted for tumour microenvironment studies as well as drug screening experiments in more complex 3D co-culture systems.
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Online measurement of oxygen enables continuous noninvasive evaluation of human-induced pluripotent stem cell (hiPSC) culture in a perfused 3D hollow-fiber bioreactor.
Selina Greuel,Nora Freyer,Güngör Hanci,Mike Böhme,Toshio Miki,Johannes Werner,Frank Schubert,Michael Sittinger,Katrin Zeilinger,Carl-Fredrik Mandenius +9 more
TL;DR: The presented results indicate that oxygen is equally as applicable as parameter for hiPSCs expansion as glucose while providing an accurate real‐time impression of hiPSC culture development.
References
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Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
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Neutral red uptake assay for the estimation of cell viability/cytotoxicity.
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Simple and Inexpensive Fluorescence-Based Technique for High-Throughput Antimalarial Drug Screening
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Related Papers (5)
Cell Viability Assays -- Assay Guidance Manual
Sittampalam Gs,Grossman A,Brimacombe K,Arkin M,Auld D,Austin C,Baell J,Bejcek B,Caaveiro Jmm,Chung Tdy,Nathan P. Coussens,Dahlin Jl,Devanaryan,Foley Tl,Glicksman M,Hall,Haas Jv,Hoare Srj,Inglese J,Iversen Pw,Kahl Sd,Stephen C. Kales,Kirshner S,Lal-Nag M,Li Z,McGee J,McManus O,Riss T,Saradjian P,Trask Oj,Weidner,Wildey Mj,Xia M,Xu X +33 more