Journal ArticleDOI
Characterization and chromosomal mapping of a human steroid 5α-reductase gene and pseudogene and mapping of the mouse homologue
Elizabeth P. Jenkins,Chih-Lin Hsieh,Athena Milatovich,Karl Normington,David M. Berman,Uta Francke,David W. Russell +6 more
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TLDR
The isolation, characterization, and chromosomal mapping of two human steroid 5 alpha-reductase genes are reported, one of which is functional, contains five exons separated by four introns, and maps to the distal short arm of chromosome 5.About:
This article is published in Genomics.The article was published on 1991-12-01. It has received 147 citations till now. The article focuses on the topics: Gene mapping & Pseudogene.read more
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Tissue distribution and ontogeny of steroid 5 alpha-reductase isozyme expression.
Anice E. Thigpen,Richard I. Silver,Joseph M. Guileyardo,M L Casey,John D. McConnell,David W. Russell +5 more
TL;DR: Results are consistent with 5 alpha-reductase type 1 being responsible for virilization in type 2-deficient subjects during puberty, and suggest that the type 2 isozyme may be an initiating factor in development of male pattern baldness.
Journal ArticleDOI
Steroid 5 alpha-reductase 2 deficiency.
TL;DR: In the 20 years since it was established that impairment of dihydrotestosterone formation is the cause of a rare form of human intersex, a wealth of information has accumulated about the genetics, endocrinology, and variable phenotypic manifestations, culminating in the cloning of cDNAs encoding two 5 alpha-reductase genes.
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Endocrine and intracrine sources of androgens in women: Inhibition of breast cancer and other roles of androgens and their precursor dehydroepiandrosterone
Fernand Labrie,Van Luu-The,Claude Labrie,Alain Bélanger,Jacques Simard,Sheng-Xiang Lin,Georges Pelletier +6 more
TL;DR: DHEA has been found to increase bone mineral density and to stimulate vaginal maturation without affecting the endometrium, while improving well-being and libido with no significant side effects and thus minimizing the potential side effects observed with androgens or estrogens administered systemically.
Journal ArticleDOI
Steroid 5α-Reductase 2 Deficiency
TL;DR: It is imperative to establish whether defects in steroid 5 alpha-reductase 2, perhaps in the heterozygous state, are responsible for a portion of cases of sporadic hypospadias, to determine whether 5alpha- reductase plays a role in progesterone action in women, and to elucidate the relation between androgen action and gender role behavior.
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Is dehydroepiandrosterone a hormone
TL;DR: The new field of intracrinology or local formation of sex steroids from DHEA in target tissues has permitted major advances in the treatment of the two most frequent cancers, namely breast and prostate cancer, while its potential use as a physiological HRT could well provide a physiological balance of androgens and estrogens, thus offering exciting possibilities for women's health at menopause.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
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Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.