Journal ArticleDOI
Characterization of a new family 75 chitosanase from Aspergillus sp. W-2.
Jianping Zhang,Hailong Cao,Shuguang Li,Yong Zhao,Wenxia Wang,Qingsong Xu,Yuguang Du,Heng Yin +7 more
TLDR
A new chitosanase gene of glycoside hydrolase (GH) family 75, csnw2, was cloned from an isolated strain Aspergillus sp.About:
This article is published in International Journal of Biological Macromolecules.The article was published on 2015-11-01. It has received 45 citations till now. The article focuses on the topics: Chitosanase & Pichia pastoris.read more
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Expression and characterization of a novel cold-adapted chitosanase suitable for chitooligosaccharides controllable preparation
TL;DR: This novel cold-adapted chitosanase provides a cleaner production process for the controllable preparation of chitooligosaccharides with the specific DP.
Journal ArticleDOI
Cloning, expression and characterization of a novel chitosanase from Streptomyces albolongus ATCC 27414
TL;DR: It is demonstrated that Csn21c can be an effective tool to produce abundant glucosamine and chitooligosaccharides (COS) from chitosan.
Journal ArticleDOI
Chitooligosaccharides display anti-tumor effects against human cervical cancer cells via the apoptotic and autophagic pathways
Mengyao Zhao,Liming Gu,Yun Li,Shumin Chen,Jiangshan You,Li-Qiang Fan,Yudong Wang,Liming Zhao +7 more
TL;DR: Experimental evidence is provided to demonstrate how the enzyme-membrane coupling reactor system can generate COS that exert bioactivity against gynecological cancers.
Journal ArticleDOI
Marine-polysaccharide degrading enzymes: Status and prospects.
TL;DR: This review describes research and prospects regarding the classification, biochemical properties, and catalytic mechanisms of the main types of marine-polysaccharide degrading enzymes, focusing on chitinase, chitosanase, alginate lyase, agarase, and carrageenase and their product oligosaccharides.
Journal ArticleDOI
Biocatalysis of Heterogenously-Expressed Chitosanase for the Preparation of Desirable Chitosan Oligosaccharides Applied against Phytopathogenic Fungi
TL;DR: Chitosanase (E.C.132) can be used to selectively produce partially-acetylated chitosans oligosaccharides (COSs), which are functional biopolymers that can widely be utilized as environmentall...
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
Koichiro Tamura,Daniel S. Peterson,Nicholas Peterson,Glen Stecher,Masatoshi Nei,Sudhir Kumar +5 more
TL;DR: The newest addition in MEGA5 is a collection of maximum likelihood (ML) analyses for inferring evolutionary trees, selecting best-fit substitution models, inferring ancestral states and sequences, and estimating evolutionary rates site-by-site.
Journal ArticleDOI
Clustal W and Clustal X version 2.0
Mark A. Larkin,Gordon Blackshields,Nigel P. Brown,R. Chenna,Paul A. McGettigan,Hamish McWilliam,Franck Valentin,Iain M. Wallace,Andreas Wilm,Rodrigo Lopez,J.D. Thompson,Toby J. Gibson,Desmond G. Higgins +12 more
TL;DR: The Clustal W and ClUSTal X multiple sequence alignment programs have been completely rewritten in C++ to facilitate the further development of the alignment algorithms in the future and has allowed proper porting of the programs to the latest versions of Linux, Macintosh and Windows operating systems.