Journal ArticleDOI
Cherenkov-excited luminescence scanned imaging
Rongxiao Zhang,Alisha V. DSouza,Jason R. Gunn,Tatiana V. Esipova,Sergei A. Vinogradov,Adam K. Glaser,L.A. Jarvis,David J. Gladstone,Brian W. Pogue +8 more
TLDR
Cherenkov-excited luminescent scanned imaging (CELSI) is introduced as a new imaging methodology utilizing 2-dimensional (∼5-mm-thick) sheets of LINAC radiation to produce Cherenkov photons, which in turn excite luminescence of probes distributed in biological tissues.Abstract:
Ionizing radiation is commonly delivered by medical linear accelerators (LINAC) in the form of shaped beams, and it is able to induce Cherenkov emission in tissue. In fluorescence-based microscopy excitation from scanned spots, lines, or sheets can be used for fast high-resolution imaging. Here we introduce Cherenkov-excited luminescence scanned imaging (CELSI) as a new imaging methodology utilizing 2-dimensional (∼5-mm-thick) sheets of LINAC radiation to produce Cherenkov photons, which in turn excite luminescence of probes distributed in biological tissues. Imaging experiments were performed by scanning these excitation sheets in three orthogonal directions while recording Cherenkov-excited luminescence. Tissue phantom studies have shown that single luminescent inclusions ∼1 mm in diameter can be imaged within 20-mm-thick tissue-like media with minimal loss of spatial resolution. Using a phosphorescent probe for oxygen, PtG4 with the CELSI methodology, an image of partial pressure of oxygen (pO2) was imaged in a rat lymph node, quantitatively restoring pO2 values in differently oxygenated tissues.read more
Citations
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Journal ArticleDOI
Recent Advances of Light-Mediated Theranostics.
TL;DR: Various light-triggered theranostic strategies and their great advances in biomedical applications in recent years are summarized and some other promising light-assisted techniques were also systemically discussed.
Journal ArticleDOI
Photonanomedicine: a convergence of photodynamic therapy and nanotechnology
Girgis Obaid,Mans Broekgaarden,Anne-Laure Bulin,Huang-Chiao Huang,Jerrin Kuriakose,Joyce F. Liu,Tayyaba Hasan,Tayyaba Hasan +7 more
TL;DR: The encouraging investigations that emphasize the potent synergy between photochemistry and nanotherapeutics, in addition to the growing realization of the value of these multi-faceted theranostic nanoplatforms, will assist in driving PNM formulations into mainstream oncological clinical practice as a necessary tool in the medical armamentarium.
Journal ArticleDOI
Cerenkov Radiation Induced Photodynamic Therapy Using Chlorin e6-Loaded Hollow Mesoporous Silica Nanoparticles.
Anyanee Kamkaew,Liang Cheng,Shreya Goel,Hector F. Valdovinos,Todd E. Barnhart,Zhuang Liu,Weibo Cai +6 more
TL;DR: An optimized system that uses Cerenkov radiation for PDT by using radionuclides to activate a well-known photosensitizer (chlorin e6, Ce6) and shows inhibition of tumor growth when mice were subcutaneously injected with [89Zr]HMSN-Ce6.
Journal ArticleDOI
Cerenkov luminescence imaging: physics principles and potential applications in biomedical sciences
Esther Ciarrocchi,Nicola Belcari +1 more
TL;DR: The physical processes that govern the production and transport in tissue of Cerenkov luminescence are described and the detectors used for CLI are presented and compared with the other optical imaging modalities sharing the same data acquisition and processing methods.
Journal ArticleDOI
Dosimetry for FLASH Radiotherapy: A Review of Tools and the Role of Radioluminescence and Cherenkov Emission
Muhammad Ramish Ashraf,Mahbubur Rahman,Rongxiao Zhang,Rongxiao Zhang,Benjamin B. Williams,Benjamin B. Williams,David J. Gladstone,David J. Gladstone,Brian W. Pogue,Brian W. Pogue,Petr Bruza +10 more
TL;DR: It is shown that luminescent detectors have a key role to play in the development of FLASH, as the field rapidly progresses toward clinical adaptation, and the unique ability of certain luminescence-based methods to provide tumor oxygenation maps in real-time with submillimeter resolution can elucidate the radiobiological mechanisms behind the FLASH effect.
References
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Cerenkov radiation and its applications
TL;DR: In this paper, a brief review of the original discovery of the radiation and the physical principles of the process that gives rise to it is given, followed by an elementary account of the theory of the effect.
Journal ArticleDOI
Prognostic significance of tumor oxygenation in humans
Sydney M. Evans,Cameron J. Koch +1 more
TL;DR: The various methods under investigation to directly or indirectly measure tissue oxygen in vivo are discussed and the advantages and disadvantages of each are considered.
Journal ArticleDOI
Dendritic phosphorescent probes for oxygen imaging in biological systems.
Artem Y. Lebedev,Andrei V. Cheprakov,Sava Sakadzic,David A. Boas,David F. Wilson,Sergei A. Vinogradov +5 more
TL;DR: A general approach to the construction of phosphorescent nanosensors with tunable spectral characteristics, variable degrees of quenching, and a high selectivity for oxygen is described.
Journal ArticleDOI
Two New “Protected” Oxyphors for Biological Oximetry: Properties and Application in Tumor Imaging
Tatiana V. Esipova,Alexander Karagodov,Joann Miller,David F. Wilson,Theresa M. Busch,Sergei A. Vinogradov +5 more
TL;DR: Two new phosphorescent probes optimized specifically for in vivo oxygen imaging by phosphorescence quenching are reported, showing high stability, reproducibility of signals, and lack of interactions with biological solutes.