Q2. What is the name of the plant?
W. somnifera, commonly known as “ashwagandha” and widely cultivated in the drier parts of India, is well known for its use in Ayurvedic medicine.
Q3. what is the methyl group of a withanolide?
The four methyl groups resonated at δ0.99 (3H, d, J = 7.0 Hz), δ0.78 (3H, s), δ1.27 (3H, s), and δ2.03 (3H, s), corresponding to the secondary methyl of C-21 and the quaternary methyls C-18, 19 and 28 of a withanolide.
Q4. What are the main uses of Withania somnifera?
Previous research supports its pharmacological uses, confirming antioxidant, anti-inflammatory (Sumantran et al., 2008), immunomodulatory, anticarcinogenic, antibacterial (Owais et al., 2005), antiparkinsonism (Sankar et al., 2007) and antistress (Geetha and Harish, 2006) properties.
Q5. How many fractions were obtained from W. somnifera?
The major withanolide in fraction E7 was obtained as a white powder yielding 3-methoxy-2,3dihydrowithaferin A (22 mg) by recystallization from CHCl3–CH3COCH3 (1:1).
Q6. What is the purpose of the manuscript?
The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form.
Q7. What was the ethyl acetate-soluble fraction of the crude extract obtained?
The ethyl acetate-soluble fraction of the crude extract acquired for this study was subjected to repeated column chromatography to yield compound 1.
Q8. What is the structure of the withanolide?
With the exception of 27-acetoxy-5β-chloro-6α-hydroxywithaferin A and withanolide Z, all the reported chlorinated withanolides contain a 6α-chloro-5β-hydroxy system.
Q9. What was the reaction of the etOAc extract?
E12 was separated over a C-18 reverse phase SPE column (5 g, 20 mL, Phenoment strata C-18), eluted with MeOH-H2O (1:1), to give a mixture of two compounds.
Q10. What was the spectra of the epoxide rings?
Normal phase TLC was performed on Sorbent Technologies Silica G TLC plates (200 μm, w/UV 254) using the solvent system CH2Cl2–EtOAc–MeOH (1:8:1), and reverse phase TLC was performed on Sorbent Technologies C18 TLC plates (150 μm, w/UV 254) using H2O-MeOH (1:1).
Q11. What was the spectra of the CDCl3 solvent?
1H and 13C spectra were recorded using the residual protonated signal in the CDCl3 solvent (δH 7.24) or the central peak of the CDCl3 triplet (δC 77.00) as the internal standard.