Journal ArticleDOI
Chromatographic method for the determination of aflatoxin M1 in cheese, yogurt, and dairy beverages.
TLDR
The results indicated that the method is adequate for the determination of AFM1 in these four types of cheese, as well as in yogurt and dairy beverages.Abstract:
The aim of this work was to develop and validate a method to determine aflatoxin M1 (AFM1) in cheese, yogurt, and dairy beverages The method consisted of aqueous methanol extraction, immunoaffinity column purification and isolation, RPLC separation, and fluorescence detection The four types of cheese samples were classified according to moisture and fat content The mean recoveries were 71% for cheese at spiked levels from 100 to 517 ng/kg, and 76% for yogurt and dairy beverages spiked at levels from 66 to 260 ng/kg The mean RSDs were 59% for cheese, and 10% for yogurt and dairy beverages The LOD was 3 ng/kg and the LOQ was 10 ng/kg for all test commodities To test the applicability of the developed method, a small survey of the presence of AFM1 in cheese, yogurt, and dairy beverages purchased in Ribeirao Preto-SP, Brazil, was conducted AFM1 was detected (> 3 ng/kg) in all samples Twenty cheese samples (83%) were contaminated with AFM1 in the range of 13-304 ng/kg In yogurt and dairy beverages, the contamination was lower (13-22 ng/kg) in five samples (42%) The results indicated that the method is adequate for the determination of AFM1 in these four types of cheese, as well as in yogurt and dairy beveragesread more
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Journal ArticleDOI
Simultaneous analysis of aflatoxins B1, B2, G1, G2, M1 and ochratoxin A in breast milk by high-performance liquid chromatography/fluorescence after liquid-liquid extraction with low temperature purification (LLE-LTP).
TL;DR: The result indicates that infants who are fed with breast milk from the milk banks are not at risk from aflatoxin and ochratoxin exposure.
Journal ArticleDOI
Aflatoxin M1 in milk and distribution and stability of aflatoxin M1 during production and storage of yoghurt and cheese
TL;DR: In this paper, the authors investigated the incidence and occurrence of aflatoxin M 1 (AFM 1 ) in Brazilian milk and infant formula and determined the distribution and stability of AFM 1 in cheese and yoghurt.
Journal ArticleDOI
Assessment of aflatoxin intake in São Paulo, Brazil
Alessandra Vincenzi Jager,M.P. Tedesco,Pollyana Cristina Maggio de Castro Souto,Carlos Augusto Fernandes de Oliveira +3 more
TL;DR: Aflatoxin consumption was assessed by aflatoxin determination in peanut, corn, bean, milk and derivatives collected directly from home of residents of Pirassununga Sao Paulo, Brazil, and a Food Frequency Questionnaire (FFQ) answered by volunteers as discussed by the authors.
Journal ArticleDOI
Determination of the aflatoxin M1 (AFM1) from milk by direct analysis in real time – mass spectrometry (DART-MS)
TL;DR: In this paper, direct analysis in real-time (DART) ionization coupled with a high resolution mass spectrometer (MS) was used for the rapid quantitative analysis of a common form of aflatoxin, AFM1, extracted from cow milk.
Journal ArticleDOI
Developments in mycotoxin analysis: an update for 2011-2012
Gordon S. Shephard,Franz Berthiller,P. A. Burdaspal,Colin Crews,M.A. Jonker,Rudolf Krska,S. MacDonald,R.J. Malone,Chris M. Maragos,Myrna Sabino,Michele Solfrizzo,H.P. van Egmond,Thomas B. Whitaker +12 more
TL;DR: Due to the rapid spread and developments in the field of multimycotoxin methods by liquid chromatography-tandem mass spectrometry, a separate section has been devoted to advances in this area of research.