Journal ArticleDOI
CIM® monolith chromatography-enhanced ELISA detection of proteins in artists' paints: Ovalbumin as a case study
Tanja Špec,Sebastijan Peljhan,Jana Vidič,Nika Lendero Krajnc,Marko Fonović,Črtomir Tavzes,Polonca Ropret +6 more
TLDR
The combined approach of ELISA and CIM® monolith-supported extraction was successfully tested also for the detection of protein in real-case paint layers, for the first time in the field of Cultural Heritage.About:
This article is published in Microchemical Journal.The article was published on 2016-07-01. It has received 8 citations till now.read more
Citations
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Journal ArticleDOI
Porous monoliths for on-line sample preparation: A review.
Jorge C. Masini,Frantisek Svec +1 more
TL;DR: The largest number of applications of molecular recognition mechanisms was observed for molecularly imprinted polymer monoliths as a consequence of the simplicity of this approach when compared to the use of immunosorbents or aptamers.
Journal ArticleDOI
Selective and sensitive glycoprotein detection via a biomimetic electrochemical sensor based on surface molecular imprinting and boronate-modified reduced graphene oxide
TL;DR: In this paper, a biomimetic electrochemical sensor based on a novel boronate-modified graphene-molecular imprinted polymers composite (BGR@MIP) was proposed for detecting glycoproteins selectively and sensitively.
Journal ArticleDOI
Use of monolithic supports for high-throughput protein and peptide separation in proteomics
TL;DR: The chromatographic conditions and the mass spectrometric data acquisition conditions are discussed and an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS‐based proteomics.
Journal ArticleDOI
Fluorescent molecularly imprinted nanoparticles with boronate affinity for selective glycoprotein detection.
Wang Yichen,Jing Luo,Xiaoya Liu +2 more
TL;DR: A biomimetic fluorescent sensor for the selective and sensitive detection of glycoprotein was developed, which was based on late-model boronate fluorescent molecularly imprinted nanoparticles (B-FMIP NPs), which exhibited linearity over the OVA concentration range and a rapid response time superior to that of other previously reported OVA sensors.
Journal ArticleDOI
Study of identification results of proteinous binding agents in Chinese painted cultural relics
Jiajia Li,Bingjian Zhang +1 more
TL;DR: Wang et al. as discussed by the authors used an enzyme-linked immunosorbent assay (ELISA) as well as immunofluorescence microscopy (IFM) to identify proteinous binders in these paintings.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Free radical-mediated oxidation of free amino acids and amino acid residues in proteins.
TL;DR: It is evident that the cyclic oxidation and reduction of the sulfur-containing amino acids may serve as an important antioxidant mechanism, and also that these reversible oxidations may provide an important mechanism for the regulation of some enzyme functions.
Book
Organic Chemistry of Museum Objects
John S. Mills,Raymond White +1 more
TL;DR: The Organic Chemistry of Museum Objects as mentioned in this paper is a survey of the chemical composition, properties and analysis of the whole range of organic materials incorporated into objects and artworks found in museum collections.
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Raman and infrared spectra of carbonates of calcite structure
TL;DR: In this article, the Raman and mid-range infrared spectra have been measured on natural limestone and dolomite minerals, and the positions of the wavenumbers are unique for each carbonate mineral and are thus diagnostic of their mineralogy.
Amorphous cellulose - structure and characterization
TL;DR: Amorphous cellulose was obtained from different types of celluloses (microcrystalline cellulose, dissolving pulp and cotton cellulose), by regeneration with ethanol from their solutions in an SO2-diethylaminedimethylsulfoxide (SO2-DEA-DMSO) solvent system as discussed by the authors.