Journal ArticleDOI
Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157:H7.
TLDR
The eae gene from enterohaemorrhagic E. coli (EHEC) which, in addition to producing Shiga‐like cytotoxins, also produces the attaching and effacing effect of EPEC infections is cloned.Abstract:
The eae (Escherichia coli attaching and effacing) gene from enteropathogenic Escherichia coli (EPEC) was previously shown to be essential for production of the 'attaching and effacing' histopathology characteristic of EPEC infections (Jerse et al., 1990). We have now cloned the eae gene from enterohaemorrhagic E. coli (EHEC) which, in addition to producing Shiga-like cytotoxins, also produces the attaching and effacing effect. The sequence homology between the EPEC and EHEC sequences was 86% and 83% at the nucleotide and amino acid levels, respectively. The predicted amino acid sequence of the EHEC eae gene shared 31% identity and 51% similarity with invasin of Yersinia pseudotuberculosis. Alignment of the EPEC and EHEC Eae proteins and the Y. pseudotuberculosis and Y. enterocolitica invasins shows striking regions of identity with the greatest divergence at the C-terminal end, the putative receptor-binding portion of invasin.read more
Citations
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Journal ArticleDOI
Diarrheagenic Escherichia coli
James P. Nataro,James B. Kaper +1 more
TL;DR: The current level of understanding of the pathogenesis of the diarrheagenic E. coli strains is discussed and how their pathogenic schemes underlie the clinical manifestations, diagnostic approach, and epidemiologic investigation of these important pathogens are described.
Journal ArticleDOI
Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections
James C. Paton,Adrienne W. Paton +1 more
TL;DR: Increased application of advanced molecular technologies in clinical laboratories has significantly improved the capacity to diagnose STEC infection early in the course of disease and to detect low levels of environmental contamination, creating a potential window of opportunity for future therapeutic intervention.
Journal ArticleDOI
Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157
Adrienne W. Paton,James C. Paton +1 more
TL;DR: Two multiplex PCR assays for the detection and genetic characterization of STEC in cultures of feces or foodstuffs detected STEC of the appropriate genotype in primary fecal cultures from five patients with hemolytic-uremic syndrome and three with bloody diarrhea.
Journal ArticleDOI
Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933.
TL;DR: The newly discovered EHEC hemolysin was shown to be responsible for the enterohemolytic phenotype and demonstrated to be related but not identical to alpha-hemolysin.
Journal ArticleDOI
Enterohaemorrhagic Escherichia coli in human medicine
TL;DR: This review will focus on the microbiology, epidemiology, and pathophysiology of EHEC-associated diseases, and illustrate future challenges and opportunities for their control.
References
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New perspectives in cell adhesion : RGD and integrins
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Escherichia coli that Cause Diarrhea: Enterotoxigenic, Enteropathogenic, Enteroinvasive, Enterohemorrhagic, and Enteroadherent
TL;DR: There are four major categories of diarrheagenic Escherichia coli: enterotoxigenic (a major cause of travelers' diarrhea and infant diarrhea in less-developed countries), enteroinvasive (a cause of dysentery), enteropathogenic (an important cause of infant diarrhea), and enterohemorrhagic ( a cause of hemorrhagic colitis and hemolytic uremic syndrome).
Journal ArticleDOI
A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.
TL;DR: The ability of enteropathogenic Escherichia coli to form attaching and effacing intestinal lesions is a major characteristic of EPEC pathogenesis and a chromosomal gene (eae) that is necessary for this activity is identified using TnphoA mutagenesis.
Journal ArticleDOI
Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector.
Michael S. Donnenberg,J B Kaper +1 more
TL;DR: The use of a novel suicide vector containing the pir-dependent R6K replicon and the sacB gene of Bacillus subtilis to construct an eae deletion mutant of EPEC is reported, establishing the utility of a new system for the construction of deletion mutations.