Co-culture of neurons and glia in a novel microfluidic platform.
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A microfluidic cell co-culture platform that permits individual manipulation of the microenvironment of different cell types and enhanced the transfection efficiency of neurons to almost 60%.About:
This article is published in Journal of Neuroscience Methods.The article was published on 2011-03-15 and is currently open access. It has received 119 citations till now. The article focuses on the topics: mCherry.read more
Citations
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Journal ArticleDOI
Microengineered physiological biomimicry: Organs-on-Chips
Dongeun Huh,Dongeun Huh,Yu Suke Torisawa,Geraldine A. Hamilton,Hyun-Jung Kim,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +7 more
TL;DR: The potential of organs-on-chips as alternatives to conventional cell culture models and animal testing for pharmaceutical and toxicology applications and the challenges that lie ahead are explored.
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Scaling and systems biology for integrating multiple organs-on-a-chip
John P. Wikswo,Erica L. Curtis,Zachary E. Eagleton,Brian C. Evans,Ayeeshik Kole,Lucas H. Hofmeister,William J. Matloff +6 more
TL;DR: This review presents several examples of scaling arguments and discusses steps that should ensure the success of this endeavour to address the formidable pharmacological and physiological gaps between monolayer cell cultures, animal models, and humans.
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Micro total analysis systems for cell biology and biochemical assays.
Michelle L. Kovarik,Philip C. Gach,Douglas M. Ornoff,Yuli Wang,Joseph Balowski,Lila Farrag,Nancy L. Allbritton +6 more
TL;DR: Application of µTAS in all of these areas continues to be highly interdisciplinary, utilizing techniques and strategies from almost every scientific field, and insures continued relevance to the biological community as well as a bright future.
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Organic electrode coatings for next-generation neural interfaces.
Ulises A. Aregueta-Robles,Andrew J. Woolley,Andrew J. Woolley,Laura A. Poole-Warren,Nigel H. Lovell,Rylie A. Green +5 more
TL;DR: The review focuses on the various organic coatings which have been investigated to improve neural interface electrodes and how they provide safe electrical stimulation of tissue while avoiding undesirable chemical reactions and cell damage.
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Organ-On-A-Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies.
Samad Ahadian,Robert A. Civitarese,Dawn Bannerman,Mohammad Hossein Mohammadi,Rick Xing Ze Lu,Erika Yan Wang,Locke Davenport-Huyer,Ben Lai,Boyang Zhang,Yimu Zhao,Serena Mandla,Anastasia Korolj,Milica Radisic +12 more
TL;DR: The latest developments in OOC platforms (e.g., liver, skeletal muscle, cardiac, cancer, lung, skin, bone, and brain) are discussed as functional tools in simulating human physiology and metabolism.
References
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Poly(dimethylsiloxane) as a material for fabricating microfluidic devices.
TL;DR: This Account summarizes techniques for fabrication and applications in biomedicine of microfluidic devices fabricated in poly(dimethylsiloxane) (PDMS).
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A microfluidic culture platform for CNS axonal injury, regeneration and transport
Anne Marion Taylor,Mathew Blurton-Jones,Seog Woo Rhee,David H. Cribbs,Carl W. Cotman,Neon Li Jeon +5 more
TL;DR: A microfluidic culture platform that polarizes the growth of CNS axons into a fluidically isolated environment without the use of targeting neurotrophins is described and the first evidence that presynaptic but not postsynaptic mRNA is localized to developing rat cortical and hippocampal axons is reported.
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Gas sorption, diffusion, and permeation in poly(dimethylsiloxane)
TL;DR: In this article, the perfluorocarbon penetrants (CF4, C2F6, and C3F8) are shown to exhibit linear sorption isotherms.
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Local control of neurite development by nerve growth factor.
TL;DR: The results show that the growth, and probably the survival, of neurites depends upon nerve growthFactor in their local environment, regardless of the nerve growth factor concentrations to which other portions of the neuron are exposed.
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Patterned cell culture inside microfluidic devices
TL;DR: The method developed in this work offers a convenient way of micropatterning biomaterials for selective attachment of cells on the substrates, and enables culturing of patterned cells inside microfluidic devices for a number of biological research applications where cells need to be exposed to well-controlled fluidic microenvironment.