Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress.
Ramesh Katam,Katsumi Sakata,Prashanth Suravajhala,Tibor Pechan,Devaiah Kambiranda,Karamthot Sivasankar Naik,Baozhu Guo,Sheikh M. Basha +7 more
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TLDR
Drought perturbs the invasion of the aflatoxin producing fungus and thus affects the quality and yield of peanut and more studies involving the effects of drought stress to determine the molecular changes will enhance the understanding of the key metabolic pathways involved in the combined stresses.About:
This article is published in Journal of Proteomics.The article was published on 2016-06-30 and is currently open access. It has received 46 citations till now. The article focuses on the topics: Plant protein & Drought tolerance.read more
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Research Progress in Membrane Lipid Metabolism and Molecular Mechanism in Peanut Cold Tolerance
He Zhang,Jiale Dong,Xinhua Zhao,Yumei Zhang,Jingyao Ren,Liting Xing,Chunji Jiang,Xiaoguang Wang,Jing Wang,Shuli Zhao,Haiqiu Yu +10 more
TL;DR: This review focuses on membrane lipid metabolism and its molecular mechanism, as well as lipid signal transduction in peanut (Arachis hypogaea L.) under cold stress to build a foundation for explicating lipid metabolism regulation patterns and physiological and molecular response mechanisms during cold stress and to promote the genetic improvement of peanut cold tolerance.
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Current Status and Future Opportunities of Omics Tools in Mycotoxin Research.
Manal Eshelli,M. Mallique Qader,M. Mallique Qader,Ebtihaj J. Jambi,Andrew Hursthouse,Mostafa E. Rateb +5 more
TL;DR: The merits of individual and combined omics approaches and their promising applications to mitigate the issue of mycotoxin contamination focus on aflatoxin, ochratoxin, and patulin.
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Physiological and Proteomic Signatures Reveal Mechanisms of Superior Drought Resilience in Pearl Millet Compared to Wheat.
Arindam Ghatak,Palak Chaturvedi,Gert Bachmann,Luis Valledor,Živa Ramšak,Mitra Mohammadi Bazargani,Prasad Bajaj,Sridharan Jegadeesan,Weimin Li,Xiaoliang Sun,Kristina Gruden,Rajeev K. Varshney,Wolfram Weckwerth +12 more
TL;DR: In this paper, a comparative physiological screening and large scale proteomics of drought stress responses in drought-tolerant and susceptible genotypes of pearl millet and wheat was performed. And the results showed that the physiological responses demonstrated large differences in the regulation of root morphology and photosynthetic machinery, revealing a stay-green phenotype in Pearl millet.
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Advances in “omics” approaches to tackle drought stress in grain legumes
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Integrated proteomics and metabolomics to unlock global and clonal responses of Eucalyptus globulus recovery from water deficit
Barbara Correia,Luis Valledor,Robert D. Hancock,Jenny Renaut,Jesús Pascual,Amadeu M.V.M. Soares,Glória Pinto +6 more
TL;DR: This study provides a set of novel proteins and pathways involved in drought stress that represent potential drought tolerance markers for early selection of Eucalyptus.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Yoshiyuki Nakano,Kozi Asada +1 more
TL;DR: Observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ASCorbate is regenerated from DHA by the system: photosystem I-*ferredoxin-*NADP^>glutathione and a preliminary characterization of the chloroplast peroxidase is given.
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Probability-based protein identification by searching sequence databases using mass spectrometry data.
TL;DR: A new computer program, Mascot, is presented, which integrates all three types of search for protein identification by searching a sequence database using mass spectrometry data, and the scoring algorithm is probability based.
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Primer3—new capabilities and interfaces
Andreas Untergasser,Ioana Cutcutache,Triinu Koressaar,Jian Ye,Brant C. Faircloth,Maido Remm,Steven G. Rozen +6 more
TL;DR: Primer3’s current capabilities are described, including more accurate thermodynamic models in the primer design process, both to improve melting temperature prediction and to reduce the likelihood that primers will form hairpins or dimers.
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Locating proteins in the cell using TargetP, SignalP and related tools
TL;DR: The properties of three well-known N-terminal sequence motifs directing proteins to the secretory pathway, mitochondria and chloroplasts are described and a brief history of methods to predict subcellular localization based on these sorting signals and other sequence properties are sketched.