Journal ArticleDOI
Comparative study of methods for quantitation of lactate dehydrogenase isozymes
TLDR
LDH-5 and LDH-4, known to be present in small amounts in rat cardiac muscle, appeared in disproportionately large amounts when too much enzyme was used for electrophoresis.About:
This article is published in Analytical Biochemistry.The article was published on 1970-08-01. It has received 79 citations till now. The article focuses on the topics: Formazan & Lactate dehydrogenase.read more
Citations
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Activities of metabolic and contractile enzymes in 18 bovine muscles.
TL;DR: Contractile and metabolic characteristics of 18 bovine muscles were studied using 10 animals of various ages, sexes, sexes (females, males and castrated males) and types and showed that myofibrillar ATPase activity was positively related to glycolytic activities and negatively related to oxidative activities.
Journal ArticleDOI
Affinity chromatography of lactate dehydrogenase Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi-substrate enzymes.
Padraig O'Carra,Standish Barry +1 more
TL;DR: Model studies on lactate dehydrogenase (LDH) demonstrate that the effectiveness of affinity chromatography as a purification tool for certain types of multi-substrate enzyme may be greatly increased by taking advantage of kinetic characteristics.
Journal ArticleDOI
Affinity chromatography of nicotinamide–adenine dinucleotide-linked dehydrogenases on immobilized derivatives of the dinucleotide
Standish Barry,Padraig O'Carra +1 more
TL;DR: It is concluded that the general effectiveness of a general ligand may be considerably distorted and attenuated by the nature of the immobilization linkage, but this attenuation can result in an increase in specific effectiveness, allowing dehydrogenases to be separated from one another in a manner unlikely to be feasible if thegeneral effectiveness of the ligand remained intact.
Journal ArticleDOI
Inadequacy of Traditional ELISA for Screening Hybridoma Supernatants for Murine Monoclonal Antibodies
TL;DR: The results of this study strongly suggest that antigen conformation on solid phase may frequently be different from in solution and that screening by ELISA in which antigen is attached to solid phase is often inappropriate for determining the presence of useful monoclonal antibodies.
Journal ArticleDOI
Lactate dehydrogenase isoenzymes.
TL;DR: The analytical procedures for LD isoenzymes include electrophoresis, chromatography, immunochemical and kinetic methods, which are used mainly for assaying acute myocardial infarction, where generally the determination of LD-1 and LD-2 is sufficient.
References
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Journal ArticleDOI
Nature and Development of Lactic Dehydrogenases: The two major types of this enzyme form molecular hybrids which change in makeup during development.
Journal ArticleDOI
Separation and quantitation of lactic dehydrogenase isoenzymes by disc electrophoresis.
A A Dietz,Tina Lubrano +1 more
TL;DR: The disc electrophoresis method of Davis does not permit the separation of all 5 isoenzymes of LDH, but the method was modified by omitting the stacking and sample gels, and substituting 40% sucrose solution.
Journal ArticleDOI
The Electrophoretically Distinct Forms of Mammalian Lactic Dehydrogenase: II. PROPERTIES AND INTERRELATIONSHIPS OF RABBIT AND HUMAN LACTIC DEHYDROGENASE ISOZYMES
Peter G. W. Plagemann,Peter G. W. Plagemann,Kenneth F. Gregory,Kenneth F. Gregory,Felix Wróblewski,Felix Wróblewski +5 more
Journal Article
Characterization of lactic dehydrogenase of normal human granulocytes.
Journal ArticleDOI
Control of synthesis of lactic acid dehydrogenases
TL;DR: It is postulated that low oxygen tensions may result in increased synthesis of messenger RNA for M subunits, and that this messenger RNA survives for at least nine hours.
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Separation and quantitation of lactic dehydrogenase isoenzymes by disc electrophoresis.
A A Dietz,Tina Lubrano +1 more