Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection.
Soraia Reda Gilber,Silvana Maria Alban,Luiza Gobor,Jessica de Oliveira Bescrovaine,Marcia Iurico Myiazaki,Vanete Thomaz-Soccol +5 more
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This work aimed to compare the current diagnostic methods for Chagas disease, including conventional serological and ELISA and molecular techniques (PCR), to introduce PCR as an auxiliary technique.Abstract:
Introduction Trypanosoma cruzi, a flagellated protozoan, is the etiologic agent of Chagas disease, and it is estimated that approximately 5 million people in Brazil are infected with this parasite. This work aimed to compare the current diagnostic methods for Chagas disease, including conventional serological (IFAT and ELISA) and molecular techniques (PCR), to introduce PCR as an auxiliary technique. Methods A total of 106 chagasic patients were evaluated: 88 from endemic areas of Parana, 6 from Sao Paulo, 3 from Minas Gerais, 3 from Rio Grande do Sul, 1 from Bahia and 5 from the Santa Catarina T. cruzi outbreak. The samples were analyzed by conventional serological methods (IFAT, ELISA), hemoculture and PCR to confirm Chagas disease. Results When IFAT was used to determine antibody levels, the sensitivity was 81.7% for patients with the cardiac form of the disease and 100% for the other clinical forms. In contrast, ELISA showed 84% sensitivity and 100% specificity. The use of serological and molecular techniques and their implications for the diagnosis of Chagas disease in non-endemics area are discussed. Conclusions PCR constitutes an excellent support methodology for the laboratory diagnosis of Chagas disease due to its high sensitivity and specificity.read more
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Chagas disease in the 21st Century: a public health success or an emerging threat?
TL;DR: As Chagas disease becomes an increasingly globalized public health issue in the twenty-first century, continued attentiveness from governmental and health organizations as well as improved diagnostic tools, expanded surveillance and increased research funding will be required to maintain existing public health successes and stymie the spread of the disease to new areas and populations.
Journal ArticleDOI
Biomarkers of therapeutic responses in chronic Chagas disease: state of the art and future perspectives
María-Jesús Pinazo,Maria-Carmen Thomas,Juan J Bustamante,Igor C. Almeida,Manuel Carlos López López,Joaquim Gascon +5 more
TL;DR: Cytokines and surface markers represent promising molecules for the characterisation of host cellular responses, but need to be further assessed for assessing responses to treatment in a short period of time.
Journal ArticleDOI
Challenges and perspectives of Chagas disease: a review.
TL;DR: This review aimed to evaluate the challenges of CD control, future perspectives, and actions performed worldwide to control expansion of the disease and its impact on public health in Latin America.
Journal ArticleDOI
Highly discordant serology against Trypanosoma cruzi in central Veracruz, Mexico: role of the antigen used for diagnostic
Daniel Guzmán-Gómez,Aracely López-Monteon,María de la Soledad Lagunes-Castro,Carolina Álvarez-Martínez,Manuel Jesús Hernández-Lutzon,Eric Dumonteil,Eric Dumonteil,Angel Ramos-Ligonio +7 more
TL;DR: Commercial tests had a very limited ability to detect T. cruzi infection in the study population, and in-house tests based on crude parasite antigens showed a greater sensitivity but were still unable to detect all cases of T. cruiser infection, even when based on a local parasite strain.
Journal ArticleDOI
Usefulness of real time PCR to quantify parasite load in serum samples from chronic Chagas disease patients.
Myllena de Fátima Alheiros Dias Melo,Otacilio C. Moreira,Priscila Tenório,Virgínia Maria Barros de Lorena,Izaura Lorena-Rezende,Wilson Alves de Oliveira Junior,Yara M. Gomes,Constança Britto +7 more
TL;DR: The comparative duplex qPCR analysis revealed that, even with an increase in Ct values, it was possible to detect all DNA targets in serum, suggesting its use in reference laboratories for the diagnosis of Chagas disease patients.
References
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TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
International study to evaluate PCR methods for detection of Trypanosoma cruzi DNA in blood samples from Chagas disease patients.
Alejandro G. Schijman,Margarita Bisio,Liliana Orellana,Mariela Sued,Tomás Duffy,Ana M. Mejia Jaramillo,Carolina Cura,Frederic Auter,Vincent Veron,Yvonne Qvarnstrom,Stijn Deborggraeve,Gisely Hijar,Inés Zulantay,Raul Horacio Lucero,Elsa F. Velazquez,Tatiana Tellez,Zunilda Sanchez Leon,Lúcia Maria da Cunha Galvão,Debbie Nolder,Maria Mercedes Monje Rumi,José Eduardo Levi,Juan David Ramírez,Pilar Zorrilla,María Flores,Maria I. Jercic,Gladys Crisante,Néstor Añez,Ana Maria de Castro,Clara Isabel González,Karla Y. Acosta Viana,Pedro Yachelini,Faustino Torrico,Carlos Robello,Patricio Diosque,Omar Triana Chávez,Christine Aznar,Graciela Russomando,Philippe Büscher,Azzedine Assal,Felipe Guhl,Sergio Sosa Estani,Alexandre J. DaSilva,Constança Britto,Alejandro O. Luquetti,Janis Ladzins +44 more
TL;DR: This study represents a first crucial step towards international validation of PCR procedures for detection of T. cruzi in human blood samples by an external quality evaluation.
Journal Article
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TL;DR: Gastrointestinal dysfunction is a major problem for many patients with chronic Chagas' disease, as are cardiac dysrhythmias and cardiomyopathy, and patients with advanced megacolon who have resections of the sigmoid colon and most of the rectum generally do well postoperatively.
Journal ArticleDOI
American Trypanosomiasis (Chagas' Disease) -- A Tropical Disease Now in the United States
TL;DR: American trypanosomiasis (Chagas' disease) is a zoonosis caused by the protozoan parasite Trypanosoma cruzi and is a major cause of morbidity and mortality among poor people in developing countries, but health care providers in industrialized nations see it less frequently and know little about it.
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