About: Universidade Positivo is a(n) education organization based out in Curitiba, Brazil. It is known for research contribution in the topic(s): Population & Biodiversity. The organization has 1290 authors who have published 1237 publication(s) receiving 9335 citation(s). The organization is also known as: Positive University.
01 Aug 2010-Bioresource Technology
TL;DR: Analysis of growth parameters, media of cultivation, biomass composition and productivity and nutrients balance, and carbon metabolism in terms of carbon dioxide fixation and its destination in microalgae cultivations found carbon dioxide fixated was mainly used for microalgal biomass production.
Abstract: The present study aimed at investigating the carbon metabolism in terms of carbon dioxide fixation and its destination in microalgae cultivations. To this purpose, analysis of growth parameters, media of cultivation, biomass composition and productivity and nutrients balance were performed. Four microalgae suitable for mass cultivation were evaluated: Dunaliella tertiolecta SAD-13.86, Chlorella vulgaris LEB-104, Spirulina platensis LEB-52 and Botryococcus braunii SAG-30.81. Global rates of carbon dioxide and oxygen were determinated by a system developed in our laboratory. B. braunii presented the highest CO(2) fixation rate, followed by S. platensis,D. tertiolecta and C. vulgaris (496.98, 318.61, 272.4 and 251.64 mg L(-1)day(-1), respectively). Carbon dioxide fixated was mainly used for microalgal biomass production. Nitrogen, phosphorus (calcium for D. tertiolecta), potassium and magnesium consumption rates (mg gX(-1)) were evaluated for the four microalgae. Biomass composition presented a predominance of proteins but also a high amount of lipids, especially in D. tertiolecta and B. braunii.
Churchill Hospital1, Wellcome Trust Centre for Human Genetics2, University of Oxford3, Universidade Positivo4, The Queen's Medical Center5, Walton Centre6, St. Michael's GAA, Sligo7, Queen's University Belfast8, Norfolk and Norwich University Hospital9, University of Birmingham10, Southern General Hospital11, Royal Hospital for Sick Children12
TL;DR: Genetic testing using targeted capture followed by next-generation sequencing was efficient, cost-effective, and enabled a molecular diagnosis in many refractory cases and has broad implications for clinical neurology practice and the approach to diagnostic testing.
Abstract: Many neurological conditions are caused by immensely heterogeneous gene mutations. The diagnostic process is often long and complex with most patients undergoing multiple invasive and costly investigations without ever reaching a conclusive molecular diagnosis. The advent of massively parallel, next-generation sequencing promises to revolutionize genetic testing and shorten the 'diagnostic odyssey' for many of these patients. We performed a pilot study using heterogeneous ataxias as a model neurogenetic disorder to assess the introduction of next-generation sequencing into clinical practice. We captured 58 known human ataxia genes followed by Illumina Next-Generation Sequencing in 50 highly heterogeneous patients with ataxia who had been extensively investigated and were refractory to diagnosis. All cases had been tested for spinocerebellar ataxia 1-3, 6, 7 and Friedrich's ataxia and had multiple other biochemical, genetic and invasive tests. In those cases where we identified the genetic mutation, we determined the time to diagnosis. Pathogenicity was assessed using a bioinformatics pipeline and novel variants were validated using functional experiments. The overall detection rate in our heterogeneous cohort was 18% and varied from 8.3% in those with an adult onset progressive disorder to 40% in those with a childhood or adolescent onset progressive disorder. The highest detection rate was in those with an adolescent onset and a family history (75%). The majority of cases with detectable mutations had a childhood onset but most are now adults, reflecting the long delay in diagnosis. The delays were primarily related to lack of easily available clinical testing, but other factors included the presence of atypical phenotypes and the use of indirect testing. In the cases where we made an eventual diagnosis, the delay was 3-35 years (mean 18.1 years). Alignment and coverage metrics indicated that the capture and sequencing was highly efficient and the consumable cost was ∼£400 (€460 or US$620). Our pathogenicity interpretation pathway predicted 13 different mutations in eight different genes: PRKCG, TTBK2, SETX, SPTBN2, SACS, MRE11, KCNC3 and DARS2 of which nine were novel including one causing a newly described recessive ataxia syndrome. Genetic testing using targeted capture followed by next-generation sequencing was efficient, cost-effective, and enabled a molecular diagnosis in many refractory cases. A specific challenge of next-generation sequencing data is pathogenicity interpretation, but functional analysis confirmed the pathogenicity of novel variants showing that the pipeline was robust. Our results have broad implications for clinical neurology practice and the approach to diagnostic testing.
TL;DR: Redistricting optimization, but not broader sharing alone, would reduce geographic disparity in allocation of livers for transplant across the United States.
Abstract: Severe geographic disparities exist in liver transplantation; for patients with comparable disease severity, 90-day transplant rates range from 18% to 86% and death rates range from 14% to 82% across donation service areas (DSAs). Broader sharing has been proposed to resolve geographic inequity; however, we hypothesized that the efficacy of broader sharing depends on the geographic partitions used. To determine the potential impact of redistricting on geographic disparity in disease severity at transplantation, we combined existing DSAs into novel regions using mathematical redistricting optimization. Optimized maps and current maps were evaluated using the Liver Simulated Allocation Model. Primary analysis was based on 6700 deceased donors, 28 063 liver transplant candidates, and 242 727 Model of End-Stage Liver Disease (MELD) changes in 2010. Fully regional sharing within the current regional map would paradoxically worsen geographic disparity (variance in MELD at transplantation increases from 11.2 to 13.5, p = 0.021), although it would decrease waitlist deaths (from 1368 to 1329, p = 0.002). In contrast, regional sharing within an optimized map would significantly reduce geographic disparity (to 7.0, p = 0.002) while achieving a larger decrease in waitlist deaths (to 1307, p = 0.002). Redistricting optimization, but not broader sharing alone, would reduce geographic disparity in allocation of livers for transplant across the United States.
Leipzig University1, Martin Luther University of Halle-Wittenberg2, Universidade Positivo3, University of Vigo4, Empresa Brasileira de Pesquisa Agropecuária5, ETH Zurich6, Moscow State University7, University of Freiburg8, University of Jena9, University of Catania10, Wageningen University and Research Centre11, Free University of Berlin12, Senckenberg Museum13, Colorado State University14, National Agriculture and Food Research Organization15, University of Nairobi16, Commonwealth Scientific and Industrial Research Organisation17, National Scientific and Technical Research Council18, Brandenburg University of Technology19, Cornell University20, University College Dublin21, United States Forest Service22, University of Toronto23, Aberystwyth University24, State University of New York at Cortland25, National University of Luján26, University of Trier27, University of the Philippines Mindanao28, Razi University29, Josip Juraj Strossmayer University of Osijek30, Kyushu University31, Minnesota Pollution Control Agency32, Aarhus University33, Northern Kentucky University34, Lincoln University (Missouri)35, University of Agricultural Sciences, Dharwad36, Fukushima University37, Matej Bel University38, Lancaster University39, Université d'Abobo-Adjamé40, Tarbiat Modares University41, Pachhunga University College42, University of São Paulo43, University of Hawaii at Hilo44, College of Tropical Agriculture and Human Resources45, Oklahoma State University–Stillwater46, Forest Research Institute47, University of Extremadura48, Katholieke Universiteit Leuven49, Research Institute for Nature and Forest50, Natural Resources Institute Finland51, University of Alcalá52, COMSATS Institute of Information Technology53, King Abdulaziz University54, University of Minnesota55, Federal University of Maranhão56, Jagiellonian University57, Technical University of Berlin58, University of Wisconsin-Madison59, Leibniz Association60, Braunschweig University of Technology61, University of Innsbruck62, Keldysh Institute of Applied Mathematics63, Russian Academy of Sciences64, Khalsa College, Amritsar65, University of La Laguna66, Kōchi University67, Universidad Pública de Navarra68, McGill University69, The Nature Conservancy70, University of Giessen71, Henan University72, University of Saint Mary73
TL;DR: It was found that local species richness and abundance typically peaked at higher latitudes, displaying patterns opposite to those observed in aboveground organisms, which suggest that climate change may have serious implications for earthworm communities and for the functions they provide.
Abstract: Soil organisms, including earthworms, are a key component of terrestrial ecosystems. However, little is known about their diversity, their distribution, and the threats affecting them. We compiled a global dataset of sampled earthworm communities from 6928 sites in 57 countries as a basis for predicting patterns in earthworm diversity, abundance, and biomass. We found that local species richness and abundance typically peaked at higher latitudes, displaying patterns opposite to those observed in aboveground organisms. However, high species dissimilarity across tropical locations may cause diversity across the entirety of the tropics to be higher than elsewhere. Climate variables were found to be more important in shaping earthworm communities than soil properties or habitat cover. These findings suggest that climate change may have serious implications for earthworm communities and for the functions they provide.
Showing all 1290 results
|Paolo R. Salvalaggio||31||92||3344|
|Vanete Thomaz Soccol||30||119||5773|
|Erika Calvano Küchler||22||184||1992|
|Susan Grace Karp||22||63||2379|
|Gisele Maria Correr||21||118||1504|
|Clóvis L. Machado-da-Silva||20||48||972|
|Carla Castiglia Gonzaga||20||153||1692|
|Juliana Feltrin de Souza||19||77||1237|
|Luiz Gustavo Lacerda||18||58||768|
|Adilson Yoshio Furuse||18||94||985|
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