Journal ArticleDOI
Composition and Structure of Sugarcane Cell Wall Polysaccharides: Implications for Second-Generation Bioethanol Production
Amanda P. De Souza,Débora C C Leite,Sivakumar Pattathil,Michael G. Hahn,Marcos Silveira Buckeridge +4 more
TLDR
In this paper, the structure and fine structure of sugarcane cell walls were analyzed using a combination of microscopic, chemical, biochemical, and immunological approaches, and the results indicate that wall architecture has important implications for the development of more efficient industrial processes for second-generation bioethanol production.Abstract:
The structure and fine structure of leaf and culm cell walls of sugarcane plants were analyzed using a combination of microscopic, chemical, biochemical, and immunological approaches. Fluorescence microscopy revealed that leaves and culm display autofluorescence and lignin distributed differently through different cell types, the former resulting from phenylpropanoids associated with vascular bundles and the latter distributed throughout all cell walls in the tissue sections. Polysaccharides in leaf and culm walls are quite similar, but differ in the proportions of xyloglucan and arabinoxylan in some fractions. In both cases, xyloglucan (XG) and arabinoxylan (AX) are closely associated with cellulose, whereas pectins, mixed-linkage-β-glucan (BG), and less branched xylans are strongly bound to cellulose. Accessibility to hydrolases of cell wall fraction increased after fractionation, suggesting that acetyl and phenolic linkages, as well as polysaccharide–polysaccharide interactions, prevented enzyme action when cell walls are assembled in its native architecture. Differently from other hemicelluloses, BG was shown to be readily accessible to lichenase when in intact walls. These results indicate that wall architecture has important implications for the development of more efficient industrial processes for second-generation bioethanol production. Considering that pretreatments such as steam explosion and alkali may lead to loss of more soluble fractions of the cell walls (BG and pectins), second-generation bioethanol, as currently proposed for sugarcane feedstock, might lead to loss of a substantial proportion of the cell wall polysaccharides, therefore decreasing the potential of sugarcane for bioethanol production in the future.read more
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Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis
Dyoni Matias de Oliveira,Aline Finger-Teixeira,Thatiane R. Mota,Victor Hugo Salvador,Flávia Carolina Moreira-Vilar,Hugo Bruno Correa Molinari,Rowan A. C. Mitchell,Rogério Marchiosi,Osvaldo Ferrarese-Filho,Wanderley Dantas dos Santos +9 more
TL;DR: The biosynthesis and roles of FA in cell wall architecture and in grass biomass recalcitrance to enzyme hydrolysis are reviewed.
Journal ArticleDOI
Unlocking the potential of lignocellulosic biomass through plant science
TL;DR: What is known about the major polymeric components of woody plant biomass is reviewed, with an emphasis on the molecular interactions that contribute to its recalcitrance to enzymatic digestion.
Journal ArticleDOI
Influence of mixed sugarcane bagasse samples evaluated by elemental and physical-chemical composition
George Jackson de Moraes Rocha,Viviane Marcos Nascimento,Adilson R. Gonçalves,Vinicius Fernandes Nunes da Silva,Carlos Martín,Carlos Martín +5 more
TL;DR: The largest producer of sugarcane in the world, Brazil, with 185 million tons of solid residues generation per harvest, is the world's largest sugar cane producing country as mentioned in this paper.
Journal ArticleDOI
Plant Fibre: Molecular Structure and Biomechanical Properties, of a Complex Living Material, Influencing Its Deconstruction towards a Biobased Composite.
TL;DR: This review provides an overview of wood as a composite material followed by its deconstruction into fibres that can then be incorporated into biobased composites.
Journal ArticleDOI
Comparative Secretome Analysis of Trichoderma reesei and Aspergillus niger during Growth on Sugarcane Biomass
Gustavo Pagotto Borin,Camila Cristina Sanchez,Amanda P. De Souza,Eliane Silva de Santana,Aline Tieppo de Souza,Adriana Franco Paes Leme,Fabio M. Squina,Marcos Silveira Buckeridge,Gustavo H. Goldman,Juliana Velasco de Castro Oliveira +9 more
TL;DR: This work provides a detailed qualitative and semi-quantitative secretome analysis of A. niger and T. reesei grown on sugarcane biomass and indicates that a combination of enzymes from both fungi is an interesting option to increase saccharification efficiency.
References
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A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy
Journal ArticleDOI
Biomass recalcitrance: engineering plants and enzymes for biofuels production.
Michael E. Himmel,Shi You Ding,David K. Johnson,William S. Adney,Mark R. Nimlos,John W. Brady,Thomas D. Foust +6 more
TL;DR: Here, the natural resistance of plant cell walls to microbial and enzymatic deconstruction is considered, collectively known as “biomass recalcitrance,” which is largely responsible for the high cost of lignocellulose conversion.
Journal ArticleDOI
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth
TL;DR: This review integrates information on the chemical structure of individual polymers with data obtained from new techniques used to probe the arrangement of the polymers within the walls of individual cells consistent with the physical properties of the wall and its components.
Journal ArticleDOI
Ethanol Production Using Corn, Switchgrass, and Wood; Biodiesel Production Using Soybean and Sunflower
David Pimentel,Tadeusz W Patzek +1 more
TL;DR: Energy outputs from ethanol produced using corn, switchgrass, and wood biomass were each less than the respective fossil energy inputs as mentioned in this paper, and the same was true for producing biodiesel using soybeans and sunflower, however, the energy cost for producing soybean biodiesel was only slightly negative compared with ethanol production.
Journal ArticleDOI
An overview of second generation biofuel technologies.
TL;DR: The biochemical route, being less mature, probably has a greater cost reduction potential than the thermo-chemical route, but here a wider range of synthetic fuels can be produced to better suit heavy truck, aviation and marine applications.