Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.
Jonathan Jarvik,David Botstein +1 more
Reads0
Chats0
TLDR
It is shown that genetic reversion of missense mutants can be of value in producing new temperature-sensitive and cold-sensitive mutations affecting related functions and it is suggested that the approach can be extended to organisms with large genomes.Abstract:
An analysis of revertants of missense mutants in phage P22 has shown: (i) New temperature-sensitive (TS) and cold-sensitive (CS) phenotypes are often acquired concomitant with reversion. (ii) In many cases, these new phenotypes are due to second-site mutations (suppressors) that correct the original defect. (iii) Sometimes the suppressor mutation is not in the same gene as the original mutation. (iv) Extragenic suppressors are almost always in genes whose products are known to interact physically with the original gene products. (v) The suppressor mutations typically retain their TS or CS phenotypes when crossed into wild-type genetic backgrounds. (vi) Some TS and CS mutants derived by reversion can themselves be reverted to produce additional mutations. We have shown that genetic reversion of missense mutants can be of value in producing new temperature-sensitive and cold-sensitive mutations affecting related functions. We suggest that our approach can be extended to organisms with large genomes.read more
Citations
More filters
Journal ArticleDOI
Protein-protein interactions: methods for detection and analysis.
Eric M. Phizicky,Stanley Fields +1 more
TL;DR: This review describes how binding affinities can be evaluated by techniques including protein affinity chromatography, sedimentation, gel filtration, fluorescence methods, solid-phase sampling of equilibrium solutions, and surface plasmon resonance.
Journal ArticleDOI
A ras-like protein is required for a post-Golgi event in yeast secretion
Antti Salminen,Peter Novick +1 more
TL;DR: It is proposed that the SEC4 product is a GTP-binding protein that plays an essential role in controlling a late stage of the secretory pathway.
Journal ArticleDOI
MCM Proteins in DNA Replication
TL;DR: New evidence suggests that the MCM2-7 proteins may be involved not only in the initiation but also in the elongation of DNA replication, which results in initiation of DNA synthesis once every cell cycle.
Journal ArticleDOI
Mapping T-cell receptor-peptide contacts by variant peptide immunization of single-chain transgenics.
TL;DR: Charge substitutions on the peptide often elicit reciprocal charges in the junctional sequences of T-cell receptor Vαor Vβ chains, indicating direct T- Cell receptor–peptide contact, and allowing derivation of a topology for the T- cell receptor–MHC interaction.
Journal ArticleDOI
Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.
Kim Nasmyth,Steven I. Reed +1 more
TL;DR: Recombinant plasmids capable of complementing cdc28 mutations were isolated by transformation of a cDC28ts strain and selection for clones capable of growth at the restrictive temperature, confirming the identity of the cloned sequences.