Journal ArticleDOI
Contribution of aerial hyphae of Aspergillus oryzae to respiration in a model solid-state fermentation system.
TLDR
In cultures of A. oryzae on a wheat-flour model substrate, aerial hyphae contributed up to 75% of the oxygen uptake rate by the fungus, which means that diffusion limitation in the densely packed mycelium layer that is formed closer to the substrate surface and that has liquid-filled pores is much less important for A.oryzae.Abstract:
Oxygen transfer is for two reasons a major concern in scale-up and process control in industrial application of aerobic fungal solid-state fermentation (SSF): 1) heat production is proportional to oxygen uptake and it is well known that heat removal is one of the main problems in scaled-up fermenters, and 2) oxygen supply to the mycelium on the surface of or inside the substrate particles may be hampered by diffusion limitation. This article gives the first experimental evidence that aerial hyphae are important for fungal respiration in SSF. In cultures of A. oryzae on a wheat-flour model substrate, aerial hyphae contributed up to 75% of the oxygen uptake rate by the fungus. This is due to the fact that A. oryzae forms very abundant aerial mycelium and diffusion of oxygen in the gas-filled pores of the aerial hyphae layer is rapid. It means that diffusion limitation in the densely packed mycelium layer that is formed closer to the substrate surface and that has liquid-filled pores is much less important for A. oryzae than was previously reported for R. oligosporus and C. minitans. It also means that the overall oxygen uptake rate for A. oryzae is much higher than the oxygen uptake rate that can be predicted in the densely packed mycelium layer for R. oligosporus and C. minitans. This would imply that cooling problems become more pronounced. Therefore, it is very important to clarify the physiological role of aerial hyphae in SSF.read more
Citations
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Journal ArticleDOI
Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: measurement and modeling.
Jos Malda,Jos Malda,Jeroen Rouwkema,Dirk E. Martens,E. P. le Comte,F.K. Kooy,Johannes Tramper,C.A. van Blitterswijk,Jens Uwe Riesle +8 more
TL;DR: The ability to measure and predict local oxygen tensions offers new opportunities to obtain more insight in the relation between oxygen tension and chondrogenesis.
Journal ArticleDOI
Species and ecological diversity within the Cladosporium cladosporioides complex (Davidiellaceae, Capnodiales)
K. Bensch,Johannes Z. Groenewald,Jan Dijksterhuis,M. Starink-Willemse,Birgitte Andersen,Brett A. Summerell,Hyeon Dong Shin,F.M. Dugan,Hans-Josef Schroers,Uwe Braun,Pedro W. Crous +10 more
TL;DR: The most important phenotypic characters for distinguishing species within the C. cladosporioides complex, which represents a monophyletic subclade within the genus, are shape, width, length, septation and surface ornamentation of conidia and conidiophores; length and branching patterns of conidial chains and hyphal shape and arrangement.
Journal ArticleDOI
The effect of PEGT/PBT scaffold architecture on oxygen gradients in tissue engineered cartilaginous constructs.
Jos Malda,Jos Malda,Tim B. F. Woodfield,F. van der Vloodt,F.K. Kooy,Dirk E. Martens,Johannes Tramper,C.A. van Blitterswijk,Jens Uwe Riesle +8 more
TL;DR: The results stress the importance of a rationally designed scaffold for tissue-engineering applications, such as the 3DF PEGT/PBT polymer scaffolds, offer possibilities for regulation of nutrient supply and, therefore, hold promise for clinical approaches for cartilage repair.
Journal ArticleDOI
Solid-state fermentation: Physiology of solid medium, its molecular basis and applications
TL;DR: This review refers to this physiology (i.e. a behavior that deviates from the one displayed by the fungus in liquid medium), sometimes referred to as “physiology of solid medium”, and its molecular basis.
Journal ArticleDOI
A review of recent developments in modeling of microbial growth kinetics and intraparticle phenomena in solid-state fermentation
TL;DR: The current article reviews the advances that has been made in both the empirical- and mechanistic-type kinetic models and discusses the insights that have been achieved through the modeling work and the improvements to models that will be necessary in the future.
References
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Journal ArticleDOI
New developments in solid state fermentation: I-bioprocesses and products.
TL;DR: SSF processes offer potential advantages in bioremediation and biological detoxification of hazardous and toxic compounds and appear to be a promising one for the production of value-added ‘low volume-high cost’ products such as biopharmaceuticals.
Journal ArticleDOI
The growth of molds in the form of pellets–a literature review
B. Metz,N. W. F. Kossen +1 more
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Localization of growth and secretion of proteins in aspergillus-niger
TL;DR: Results indicate that glucoamylase secretion is located at the tips of growing hyphae only, and that Aspergillus niger growth and secretion are confined to the periphery of colonies.
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Oxygen Microelectrode That Is Insensitive to Medium Chemical Composition: Use in an Acid Microbial Mat Dominated by Cyanidium caldarium.
TL;DR: A novel oxygen microelectrode with a tip diameter of 2 to 20 mum was constructed which could function satisfactorily under a variety of environmental conditions and in a range of media, including human blood serum, citric acid at pH 2.5, moist air, and paraffin oil.
Journal ArticleDOI
Temperature control in a continuously mixed bioreactor for solid‐state fermentation
TL;DR: This work shows that continuous mixing provides promising possibilities for simultaneous control of temperature and moisture content in solid-state fermentation on a large scale and reduces the need for evaporative cooling in the paddle mixer.