Journal ArticleDOI
Controlled Site-Directed Assembly of Antibodies by Their Oligosaccharide Moieties onto APTES Derivatized Surfaces.
Qian Weiping,Xu Bin,Wu Lei,Wang Chunxiao,Yao Danfeng,Yu Fang,Yuan Chunwei,Wei Yu +7 more
- Vol. 214, Iss: 1, pp 16-19
TLDR
A convenient and efficient method for the site-directed incorporation of aldehydes generated on the oligosaccharide moieties at the C-terminal of immunoglobulin (IgG) using NaIO4 oxidation reaction is explored.Abstract:
A convenient and efficient method for the site-directed incorporation of aldehydes generated on the oligosaccharide moieties at the C-terminal of immunoglobulin (IgG) using NaIO4oxidation reaction is explored as a means of ensuring controlled assembly of IgG antibodies onto aminopropyltriethoxylsilane (APTES) derivatized silicon wafer surfaces. The orientation and antigen binding capacity (AgBC) of site-directly assembled IgG antibodies on derivatized surfaces were investigated using atomic force microscopy (AFM) and enzyme immunoassay (EIA), respectively. A major difference in preferential orientation is observed when the incubation of derivatized surfaces with oxidized IgG molecules is compared in two different kinds of buffer solutions. We obtained the stable and homogeneous IgG layer without loss of the AgBC on the APTES derivatized surface using the controlled incubation condition.read more
Citations
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Journal ArticleDOI
Optimizing antibody immobilization strategies for the construction of protein microarrays.
Paul Peluso,David S. Wilson,Duc Do,Huu Tran,Maanasa Venkatasubbaiah,David Quincy,Bettina Heidecker,Kelli Poindexter,Neil Tolani,Michael L. Phelan,Krista Witte,Linda S Jung,Peter Wagner,Steffen Nock +13 more
TL;DR: The specific orientation of capture agents consistently increases the analyte-binding capacity of the surfaces, with up to 10-fold improvements over surfaces with randomly oriented capture agents.
Journal ArticleDOI
Microfluidic Enzyme Immunoassay Using Silicon Microchip with Immobilized Antibodies and Chemiluminescence Detection
Julia Yakovleva,Richard Davidsson,Anna Lobanova,Martin Bengtsson,Sergei A. Eremin,Thomas Laurell,Jenny Emnéus +6 more
TL;DR: The buffer composition was shown to have a dramatic effect on the assay stability, where the commonly used phosphate buffer saline was proved to be the least suitable choice.
Journal ArticleDOI
Optimization and characterization of biomolecule immobilization on silicon substrates using (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde linker
TL;DR: It is observed that a uniform, homogenous and highly dense layer of biomolecules are immobilized with optimized silane layer on the silicon substrate.
Journal ArticleDOI
XPS study on the use of 3-aminopropyltriethoxysilane to bond chitosan to a titanium surface.
TL;DR: Using toluene to bond APTES on titanium surfaces, rather than aqueous solutions, prevented the formation of unwanted polysiloxanes and increased the amount of silane on the surface for forming bonds to the chitosan films.
Journal ArticleDOI
Structure and Growth of Chromophore-Functionalized (3-Aminopropyl)triethoxysilane Self-Assembled on Silicon
TL;DR: In this paper, the authors used X-ray reflectivity, ellipsometry, and Xray photoelectron spectroscopy to characterize self-assembled films of (3-aminopropyl)triethoxysilane (APS) functionalized with a photosensitive chromophore.
References
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Covalent Attachment of Cell-Adhesive, (Arg-Gly-Asp)-Containing Peptides to Titanium Surfaces
TL;DR: A three-step reaction procedure was applied to introduce RGD-containing peptides on the titanium surface, and an approximate coverage of 0.2∼0.4 peptides/nm2 was calculated.
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TL;DR: In this paper, the authors discuss recent advances in biosensor technology which draw on the disciplines of physics, chemistry, biochemistry and electronics, and comment briefly on practical factors affecting the commercialization of various biosensors.