Journal ArticleDOI
Copper stress underlies the fundamental change in intracellular location of methane mono-oxygenase in methane-oxidizing organisms: Studies in batch and continuous cultures
TLDR
Results indicated that the copper effect could explain a similar switch in intracellular location observed in Methylosinus trichosporium OB3b but that some methanotrophs do not have the capacity to overcome copper stress in this way.Abstract:
The intracellular location of methane mono-oxygenase (MMO) activity in the methanotroph Methylococcus capsulatus (Bath) has been shown to depend primarily on the availability of copper. MMO activity was observed in the particulate fraction of cell extracts under conditions of copper excess but switched to a soluble location in response to copper stress. The two activities could be differentiated by sensitivity to a range of inhibitors and by major changes in the polypeptide banding patterns on denaturing polyacrylamide gels. MMO activity concomitant with the oxidation of ethanol was only observed in cells with particulate MMO activity but could be lost independently in response to copper stress. Examination of other methanotrophs indicated that the copper effect could explain a similar switch in intracellular location observed in Methylosinus trichosporium OB3b but that some methanotrophs do not have the capacity to overcome copper stress in this way.read more
Citations
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Journal ArticleDOI
Geometric and Electronic Structure/Function Correlations in Non-Heme Iron Enzymes
Edward I. Solomon,Thomas C. Brunold,Mindy I. Davis,Jyllian N. Kemsley,Sang-Kyu Lee,Nicolai Lehnert,Frank Neese,Andrew J. Skulan,Yi-Shan Yang,Jing Zhou +9 more
TL;DR: A detailed molecular mechanism has been proposed for IPNS based on spectroscopic and crystallographic studies and the role of cosubstrate ascorbate is proposed to reduce the toxic peroxo byproduct to water.
Journal ArticleDOI
Copper Active Sites in Biology
Edward I. Solomon,David E. Heppner,Esther M. Johnston,Jake W. Ginsbach,Jordi Cirera,Munzarin F. Qayyum,Matthew T. Kieber-Emmons,Christian H. Kjaergaard,Ryan G. Hadt,Li Tian +9 more
TL;DR: This review presents in depth discussions of all these classes of Cu enzymes and the correlations within and among these classes, as well as the present understanding of the enzymology, kinetics, geometric structures, electronic structures and the reaction mechanisms these have elucidated.
Journal ArticleDOI
Evidence that participate methane monooxygenase and ammonia monooxygenase may be evolutionarily related
TL;DR: Analysis of predicted amino acid sequences of these genes revealed strong conservation of both primary and secondary structure, suggesting that the particulate methane monooxygenase and ammonia mono Oxygenase are evolutionarily related enzymes despite their different physiological roles in these bacteria.
Journal ArticleDOI
Methanotrophs and copper
TL;DR: The current state of knowledge of the phylogeny, environmental distribution, and potential applications of methanotrophs for regional and global issues are summarized, as well as the role of Cu in regulating gene expression and proteome in these cells, its effects on enzymatic and whole-cell activity, and the novel Cu uptake system used by methanOTrophs are summarized.
Journal ArticleDOI
Microbial methane oxidation processes and technologies for mitigation of landfill gas emissions.
Charlotte Scheutz,Peter Kjeldsen,Jean E. Bogner,Alex De Visscher,Julia Gebert,Helene Hilger,Marion Huber-Humer,Kurt A. Spokas +7 more
TL;DR: Additional research and technology development is needed before methane mitigation technologies utilizing microbial methane oxidation processes can become commercially viable and widely deployed.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Enrichment, isolation and some properties of methane-utilizing bacteria.
TL;DR: The organisms were classified into five groups on the basis of morphology, fine structure, and type of resting stage formed (exospores and different types of cysts) and into subgroups on other properties.
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The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds.
TL;DR: Methane mono-oxygenase of Methylococcus capsulatus (Bath) catalyses the oxidation of various substituted methane derivatives including methanol and, in some of its catalytic properties, apparently resembles the analogous enzyme from Methylomonas methanica but differs from those found in Methylosinus trichosporium and M methylomonas albus.
Journal ArticleDOI
Resolution of the methane mono-oxygenase of Methylococcus capsulatus (Bath) into three components. Purification and properties of component C, a flavoprotein
TL;DR: Ion-exchange chromatography resolves the methane mono-oxygenase from soluble extracts of Methylococcus capsulatus (Bath) into three fractions that are comparatively stable at 0 degrees C, whereas fraction C is very unstable unless kept in the presence of sodium thioglycollate or dithiothreitol.