Journal ArticleDOI
Cyanobacterial H2 production - a comparative analysis
Kathrin Schütz,Thomas Happe,Olga Troshina,Olga Troshina,Peter Lindblad,Elsa Leitão,Paulo J. Oliveira,Paula Tamagnini +7 more
TLDR
It was shown that the hydrogen uptake activity is linked to the nitrogenase activity, whereas the hydrogen evolution activity of the bidirectional hydrogenase is not dependent or even related to diazotrophic growth conditions.Abstract:
Several unicellular and filamentous, nitrogen-fixing and non-nitrogen-fixing cyanobacterial strains have been investigated on the molecular and the physiological level in order to find the most efficient organisms for photobiological hydrogen production. These strains were screened for the presence or absence of hup and hox genes, and it was shown that they have different sets of genes involved in H2 evolution. The uptake hydrogenase was identified in all N2-fixing cyanobacteria, and some of these strains also contained the bidirectional hydrogenase, whereas the non-nitrogen fixing strains only possessed the bidirectional enzyme. In N2-fixing strains, hydrogen was mainly produced by the nitrogenase as a by-product during the reduction of atmospheric nitrogen to ammonia. Therefore, hydrogen production was investigated both under non-nitrogen-fixing conditions and under nitrogen limitation. It was shown that the hydrogen uptake activity is linked to the nitrogenase activity, whereas the hydrogen evolution activity of the bidirectional hydrogenase is not dependent or even related to diazotrophic growth conditions. With regard to large-scale hydrogen evolution by N2-fixing cyanobacteria, hydrogen uptake-deficient mutants have to be used because of their inability to re-oxidize the hydrogen produced by the nitrogenase. On the other hand, fermentative H2 production by the bidirectional hydrogenase should also be taken into account in further investigations of biological hydrogen production.read more
Citations
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Journal ArticleDOI
Occurrence, classification, and biological function of hydrogenases: an overview.
Journal ArticleDOI
Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases
Journal ArticleDOI
Activation and Inactivation of Hydrogenase Function and the Catalytic Cycle: Spectroelectrochemical Studies
Journal ArticleDOI
[NiFe] and [FeFe] hydrogenases studied by advanced magnetic resonance techniques.
Journal ArticleDOI
Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms.
Maria L. Ghirardi,Matthew C. Posewitz,Pin-Ching Maness,Alexandra Dubini,Jianping Yu,Michael Seibert +5 more
TL;DR: The photobiological production of H2 gas, using water as the only electron donor, is a property of two types of photosynthetic microorganisms: green algae and cyanobacteria, which contains only one of two major types of hydrogenases, [FeFe] or [NiFe] enzymes, which are phylogenetically distinct but perform the same catalytic reaction, suggesting convergent evolution.
References
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Journal ArticleDOI
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