Journal ArticleDOI
Cyclin synthesis drives the early embryonic cell cycle.
TLDR
In this paper, the addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles and the newly synthesized cyclin protein accumulates during each interphase and is degraded at the end of each mitosis.Abstract:
We have produced extracts of frog eggs that can perform multiple cell cycles in vitro. Destruction of the endogenous messenger RNA arrests the extracts in interphase. The addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles. The newly synthesized cyclin protein accumulates during each interphase and is degraded at the end of each mitosis.read more
Citations
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Journal ArticleDOI
Universal control mechanism regulating onset of M-phase
TL;DR: The onset of M-phase is regulated by a mechanism common to all eukaryotic cells and requires p34cdc2 dephosphorylation and association with cyclin.
Journal ArticleDOI
Cyclin is degraded by the ubiquitin pathway
TL;DR: Cyclin degradation is the key step governing exit from mitosis and progress into the next cell cycle, and anaphase may be triggered by the recognition of cyclin by the ubiquitin-conjugating system.
Journal ArticleDOI
Cyclin D1 is a nuclear protein required for cell cycle progression in G1.
TL;DR: Results demonstrate that cyclin D1 is a critical target of proliferative signals in G1, a putative G1 cyclin, in normal diploid human fibroblasts.
Journal ArticleDOI
Cyclin A is required at two points in the human cell cycle.
TL;DR: It is found that DNA synthesis and entry into mitosis are inhibited in human cells microinjected with anti‐cyclin A antibodies at distinct times, suggesting that cyclin A defines novel control points of the human cell cycle.
References
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Journal ArticleDOI
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
Yoshio Masui,Clement L. Markert +1 more
TL;DR: Fully grown oocytes of the frog (Rana pipiens) undergo cytoplasmic and nuclear maturation when treated with progesterone after the follicular envelopes have been removed, and the arrest of mitosis and cleavage can be attributed to a specific “cytostatic factor” in the cy toplasm of the secondary oocyte.
Journal ArticleDOI
Cyclin: A protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division
TL;DR: Eggs of the sea urchin Lytechinus pictus and oocytes of the surf clam Spisula solidissima also contain proteins that only start to be made after fertilization and are destroyed at certain points in the cell division cycle, and it is proposed to call these proteins the cyclins.
Journal ArticleDOI
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
TL;DR: It is shown here that a plasmid containing a cloned gene coding for a yeast leucine tRNA comes under developmental control when injected into cleaving eggs, suggesting that the MBT is triggered by the DNA through titration of suppressor components present in the egg.
Journal ArticleDOI
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity
TL;DR: It is shown that cyclin plays a pivotal role in the control of mitosis and a proteolysis-resistant mutant of cyclin prevents the inactivation of maturation promoting factor and the exit from mitosis both in vivo and in vitro.
Journal ArticleDOI
cdc25+ functions as an inducer in the mitotic control of fission yeast.
Paul Russell,Paul Nurse +1 more
TL;DR: Evidence is described showing that cdc25+ functions to counteract the activity of the mitotic inhibitor wee1+, and indicating that both mitotic control elements act independently to regulate the initiation of mitosis.