Journal ArticleDOI
Cytochemical localization and antigenicity of α-amylase in barley aleurone tissue.
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TLDR
In GA3-treated tissue, the amylase distribution was much more diffuse, although patchy, throughout the cytoplasm and it tended to accumulate in the endosperm side of the cell.Abstract:
Gibberellic-acid(GA3)-induced α-amylase has been localised in barley aleurone layers using cytochemical methods and light microscopy. Evidence obtained from the use of a starch substrate film method as well as immunofluorescence indicated that the first amylase to appear in the cell was associated with aleurone grains, apparently with the outer membrane, and also with the peripheral cytoplasm. In GA3-treated tissue, the amylase distribution was much more diffuse, although patchy, throughout the cytoplasm and it tended to accumulate in the endosperm side of the cell. The possibility that the aleurone grain membrane is the site of gibberellin-induced enzyme synthesis and that it proliferates to become rough endoplasmic reticulum is considered. Immunological information was obtained which supports earlier indications that induced α-amylase consists of two different proteins, each with molecular heterogeneity.read more
Citations
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Book ChapterDOI
The Seed: Germination
TL;DR: The nature of the pre-quiescence embryo bears very heavily on early germination behaviour and its control, and answers to questions relating to Germination behaviour are increasingly being sought during the period of seed development.
Journal ArticleDOI
Gibberellic acid enhances the level of translatable mRNA for α-amylase in barley aleurone layers
TL;DR: Stimulation could be detected about 4 h after hormone treatment and although GA3 probably stimulates synthesis of a limited number of proteins, there was stimulation of a wide size range of poly(A)-containing RNAs, so it was not possible to say that GA3 had a selective effect on the synthesis of the mRNA for α-amylase.
Journal ArticleDOI
Enzymic mechanisms of starch breakdown in germinating rice seeds: 7. Amylase formation in the epithelium
Kazuo Okamoto,Takashi Akazawa +1 more
TL;DR: The time sequence analysis of the starch digestion pattern of the thin sectioned germinating rice (Oryza sativa L.) seed specimens using the starch film method showed that at the initial stage amylase activity was almost exclusively localized in the epithelium septum between the scutellum and endosperm.
Journal ArticleDOI
Protein bodies of seeds: Ultrastructure, biochemistry, biosynthesis and degradation
TL;DR: Protein bodies may be considered as being an example of tonoplast origin from endo-plasmic reticulum, which is based mainly on storage proteins and phytic acid but, hydrolytic enzymes, cations and ribonucleic acids are also present.
Journal ArticleDOI
A Spatial Analysis of Physiological Changes Associated with Infection of Cotyledons of Marrow Plants with Cucumber Mosaic Virus.
TL;DR: Changes in host primary metabolism associated with the compatible interaction between cucumber mosaic virus and cotyledons of the marrow plant (Cucurbita pepo L.) have been localized by measuring activities of key enzymes in infected and uninfected regions of the cotYledon, and by histochemical techniques applied to tissue prints of the infected region.
References
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Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Gibberellic Acid-Enhanced Synthesis and Release of α-Amylase and Ribonuclease by Isolated Barley and Aleurone Layers
TL;DR: Gibberellic acid enhances the synthesis of alpha-amylase in isolated aleurone layers of barley-seeds and enhances the formation of ribonuclease which increases linearly over a 48 hour period.
Journal ArticleDOI
A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.
Philip Filner,Joseph E. Varner +1 more
Journal ArticleDOI
The glycogen-amylase complex as a means of obtaining highly purified α-amylases
Abraham Loyter,Michael Schramm +1 more
TL;DR: In this article, a method for the removal of glycogen from the complex, with the subsequent preparation of the crystalline pancreatic and parotis enzymes, is described, and the specific activity (units/mg protein) of enzymes thus precipitated approaches or is equal to that of the best crystalleine prepations.