Journal ArticleDOI
Detection and Classification of Mosaic Virus Disease in Cassava Plants by Proximal Sensing of Photochemical Reflectance Index
Sadasivan Nair Raji,Narayanan Subhash,V Ravi,R. Saravanan,Changatharayil N. Mohanan,T. Makeshkumar,Sukumar Nita +6 more
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TLDR
In this article, the authors investigated the application of photochemical reflectance index (PRI) imaging to detect and assess the impact of varying levels of CMD infection in cassava.Abstract:
Cassava Mosaic virus Disease (CMD) is the most severe and widespread virus infection that affects cassava (Manihot esculenta Crantz) crops. This paper investigates the application of photochemical reflectance index (PRI) imaging to detect and assess the impact of varying levels of CMD infection in cassava. Towards this, narrow band reflectance images of field-grown cassava plants were recorded at 531 and 571 nm by proximal sensing with a multispectral imaging system (MSIS). It was observed that the PRI values increase with increasing levels of CMD infection in all the varieties of cassava studied. A scatter plot of the PRI image intensity yielded a sensitivity of 85 % and specificity of 79 % for discriminating visibly no CMD from initial CMD and a sensitivity of 93 % and specificity of 92 % for discriminating initial CMD from advanced CMD. Area under the receiver operator characteristics (AUC-ROC) curve was used to discriminate the CMD infection level by differentiating visibly no CMD from initial CMD [AUC = 0.92] and initial CMD from advanced CMD [AUC = 0.99]. It was observed that PRI values determined from the experimental data follow a linear inverse relationship with net photosynthetic rate (Pn) (R
2 = 0.76) and total leaf chlorophyll (Chl) content (R
2 = 0.80). The results show that PRI imaging can be utilized to discriminate healthy plants from CMD and other stress infected crops by proximal sensing in outdoor plants.read more
Citations
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High throughput phenotyping to accelerate crop breeding and monitoring of diseases in the field
TL;DR: Recent advances in high throughput field phenotyping technologies designed to inform the genetics of quantitative traits, including crop yield and disease tolerance are reviewed.
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Detecting the severity of maize streak virus infestations in maize crop using in situ hyperspectral data
TL;DR: The potential of remotely sensed data in the accurate detection of food crop diseases such as MSV and their severity is underscores, which is critical in crop monitoring to foster food security especially in the resource-limited sub-Saharan Africa.
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Active and Passive Electro-Optical Sensors for Health Assessment in Food Crops
Thomas Fahey,Hai Pham,Alessandro Gardi,Roberto Sabatini,D. Stefanelli,Ian Goodwin,David William Lamb +6 more
TL;DR: In this paper, a review examines the key sensor characteristics, platform integration options and data analysis techniques recently proposed in the field of precision agriculture and highlights the key challenges and benefits of each concept towards informing future research.
Journal Article
Elimination of Cassava Mosaic Disease through Meristem Culture and Field Evaluation for Yield Loss Assessment in Cassava Genotypes
D C Deepthi,T Makeshkumar +1 more
TL;DR: Meristem culture for cleaning of five different cassava cultivars infected with SLCMV is reported and meristem derived plantlets were free of symptoms and the absence of virus was confirmed through PCR using virus specific primers.
Journal ArticleDOI
Combination of an image-posting system and molecular diagnosis for detecting Sri Lankan cassava mosaic virus
TL;DR: To diagnose a pathogen, novel PCR primers for CMD identification were designed and SLCMV occurrence in Kampong Thom, Cambodia was detected.
References
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A Re-Examination of the Relative Turgidity Technique for Estimating Water Deficits in Leaves
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TL;DR: The relative turgidity technique as discussed by the authors compares the initial and turgid water contents, on a percentage basis, of disks punched from leaves, the turgity water content being obtained by floating the disks on water.
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A narrow-waveband spectral index that tracks diurnal changes in photosynthetic efficiency
TL;DR: In this article, the authors presented a new "physiological reflectance index" (PRI) isolated from narrow waveband spectral measurements of sunflower canopies, which correlates with the epoxidation state of the xanthophyll cycle pigments.
Journal ArticleDOI
The role of xanthophyll cycle carotenoids in the protection of photosynthesis
TL;DR: Recent advances in the understanding of the photophysics, biochemical regulation and ecophysiology of this essential photoprotective process are reviewed.
Journal ArticleDOI
Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis.
Wolfgang Bilger,Olle Björkman +1 more
TL;DR: Measurements of light-induced absorbance changes, Chl fluorescence, and photosynthetic O2 evolution in sun and shade leaves of Hedera canariensis support the conclusion that the xanthophyll cycle has a central role in regulating the energy flow to the PS II reaction centers and also provide direct evidence that zeaxanthin protects against photoinhibitory injury to the photosynthesis system.