Journal ArticleDOI
Detection of Australia antigen and antibody by means of radioimmunoassay techniques.
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TLDR
It has been shown that the presence of anti-Au does not always prevent the development of hepatitis, and it has been possible to identify Au in some but not all units of blood that have induced hepatitis.Abstract:
Australia antigen (Au) has been identified in the blood of patients with "long-incubation hepatitis" by use of agar-gel diffusion or complement fixation (CF) [1-6]. Characterization of the antigen as a viruslike particle and its localization in the nuclei of liver cells of patients with hepatitis have provided evidence that Au may be the agent of long-incubation hepatitis [7-11]. A precipitating and complement-fixing antibody to Au (anti-Au) has been identified in the serum of patients who have received multiple transfusions but rarely, if ever, in serum from patients convalescing from hepatitis [1-3, 5-12]. It has been shown that the presence of anti-Au does not always prevent the development of hepatitis [13]. Transfusion of blood containing Au may result in hepatitis in the recipient [3, 12]. With agar-gel diffusion, it has been possible to identify Au in some but not all units of blood that have inducedread more
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Journal ArticleDOI
Vertical transmission of hepatitis B antigen in Taiwan.
TL;DR: It is indicated that vertical transmission from carrier mothers frequently occurs, at least in Taiwan, and may partially explain Taiwan's high prevalence of HB5 Ag.
Patent
Process for the demonstration and determination of reaction components having specific binding affinity for each other
TL;DR: In this article, a process for the determination of a component of the reaction between a specific binding protein and the substance being specifically bound by such a protein comprising reacting the component to be determined with its binding partner in an insolubilized form, separating the solid phase of a reaction mixture from the liquid phase, reacting with a determined amount of a coupling product of the substance, and finally determining the enzyme activity of the liquid or solid phase obtained.
Journal ArticleDOI
Hemagglutination Assay for Antigen and Antibody Associated with Viral Hepatitis
Girish N. Vyas,N. R. Shulman +1 more
TL;DR: The hemagglutination test has the sensitivity and rapidity of the best tests available, is simpler to perform, and lends itself to large-scale screening of blood donors.
Journal ArticleDOI
Viral Hepatitis: New Light on an Old Disease
Saul Krugman,Joan P. Giles +1 more
TL;DR: Hepatitis-associated antigen was consistently present in sera from patients with MS-2 strain of serum hepatitis (SH); it was not present in MS-1, infectious hepatitis (IH), and Gamma-globulin consistently neutralized the infectivity of IH (MS-1) serum; in most cases it did not neutralize the infectivities of SH (MS -2) serum.
References
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Journal ArticleDOI
Virus-like Particles in Sera of Patients With Infectious and Serum Hepatitis
TL;DR: Electron microscopy revealed morphologically similar virus-like particles both in sera from patients with hepatitis and from healthy apes, and a single virus group may be responsible for both infectious and serum hepatitis.
Journal ArticleDOI
Australia Antigen and Acute Viral Hepatitis
TL;DR: Australia antigen (Au(1)) was found in the sera of 20% of 125 patients with acute viral hepatitis and it was not present in theSera of 138 patients with other diseases affecting the liver.
Journal ArticleDOI
Biophysical Properties of Australia Antigen
John L. Gerin,John L. Gerin,Robert H. Purcell,Robert H. Purcell,M D Hoggan,M D Hoggan,Paul V. Holland,Paul V. Holland,Robert M. Chanock,Robert M. Chanock +9 more
TL;DR: Biophysical studies with Australia complement-fixing antigen showed it to be a particle with a buoyant density of 1.20 g/cm(3) in CsCl, a sedimentation coefficient of 110, and an average diameter of 25 nm.
Journal ArticleDOI
Australia antigen detected in the nuclei of liver cells of patients with viral hepatitis by the fluorescent antibody technic.
TL;DR: The appearance of purified fractions when viewed with the electron microscope was the most startling findings in the investigation of Au(1), and both the human and rabbit antisera gave identical precipitin reactions.
Journal ArticleDOI
Failure of australia antibody to prevent post-transfusion hepatitis
TL;DR: The occurrence of hepatitis in patients with antibody to the Australia antigen suggests that such antibody does not completely protect against hepatitis, or, alternatively, that the hepatitis was caused by an agent serologically distinct from Australia antigen.
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