Book ChapterDOI
Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants
TLDR
Methods for determining total ROS and lipid peroxidation assay, histochemical staining techniques for superoxide and H( 2)O(2) molecules are described.Abstract:
Reactive oxygen species or intermediates are formed by the incomplete reduction of oxygen. Organisms living in aerobic environment generate various kinds of reactive oxygen species (ROS) molecules, such as superoxide (*O(2)(-)), hydrogen peroxide (H(2)O(2)), hydroxyl radical (OH(-)), singlet oxygen, and lipid hydroperoxides. ROS are highly reactive molecules and are extremely unstable, so detection of ROS relies on measuring the end products that are formed when they react with particular substances. The end products can be measured by changes in their fluorescence, color, or luminescence. ROS causes lipid peroxidation wherein the lipids in the cell membranes are damaged. Lipid peroxidation is usually quantified using a colorimetric assay. When ROS concentrations reach a certain threshold, it activates a programmed cell death response in the cells. This is quantified by measuring the amount of ion leakage. ROS such as superoxide and hydrogen peroxide have been detected traditionally by staining techniques. Superoxide anion is detected with nitroblue tetrazolium (NBT) and hydrogen peroxide by Diaminobenzidine tetrahydrochloride (DAB) staining. In this chapter, methods for determining total ROS and lipid peroxidation assay, histochemical staining techniques for superoxide and H(2)O(2) molecules are described.read more
Citations
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Journal ArticleDOI
Increased drought stress resilience of maize through endophytic colonization by Burkholderia phytofirmans PsJN and Enterobacter sp. FD17
TL;DR: The data suggest that maize plants can be protected from inhibitory effects of the drought stress by the harbored bacterial endophytes, although the degree of protection depends on the type of the bacterial strain and the plant genotype.
Journal ArticleDOI
Drought stress amelioration in wheat through inoculation with Burkholderia phytofirmans strain PsJN
Muhammad Naveed,Muhammad Naveed,M. Baqir Hussain,Zahir Ahmad Zahir,Birgit Mitter,Angela Sessitsch +5 more
TL;DR: Inoculation of wheat with PsJN significantly diluted the adverse effects of drought on relative water contents and CO2 assimilation rate thus improving the photosynthetic rate, water use efficiency and chlorophyll content over the uninoculated control.
Journal ArticleDOI
Confident performance of chitosan and pistachio shell biochar on reducing Ni bioavailability in soil and plant plus improved the soil enzymatic activities, antioxidant defense system and nutritional quality of lettuce.
TL;DR: Results suggested that the CN2% treatment performed excellently in terms of reducing Ni concentrations in leaves and roots of lettuce plants along bioavailable Ni in the soil after plant harvest, whereas, application of BR at 2% could remarkably develop aforementioned parameters in lettuce plants.
Journal ArticleDOI
Potential of pistachio shell biochar and dicalcium phosphate combination to reduce Pb speciation in spinach, improved soil enzymatic activities, plant nutritional quality, and antioxidant defense system
TL;DR: Results depicted that the highest reduction in DTPA-extractable Pb and the concentrations of Pb in shoots and roots was achieved in DCP1%+BH1% treatment that was up to 58%, 66%, and 53%, respectively over control.
Journal ArticleDOI
Physiological and Molecular Response of Arabidopsis thaliana (L.) to Nanoparticle Cerium and Indium Oxide Exposure
TL;DR: Exposure at 250 ppm CeO2 NPs significantly increased plant biomass, but at 500–2000 ppm, plant growth was decreased by up to 85% in a dose-dependent fashion, and total chlorophyll and MDA production were unaffected by In2O3 NPs exposure.
References
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Journal ArticleDOI
Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase
Journal ArticleDOI
Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction
TL;DR: 3,3-diaminobenzidine polymerizes instantly and locally as soon as it comes into contact with H2O2 in the presence of peroxidase, and it was found that, by allowing the leaf to take up this substrate, in-vivo and in-situ detection of H2 O2 can be made at subcellular levels.
Journal ArticleDOI
Initiation of Runaway Cell Death in an Arabidopsis Mutant by Extracellular Superoxide
TL;DR: Runaway cell death seen in lsd1 plants reflected abnormal accumulation of superoxide and lack of responsiveness to signals derived from it.
Journal ArticleDOI
Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components
TL;DR: Results indicate that an O 2 − -generating system may be activated in potato tissues during the incompatible interaction induced by invading fungi or fungal wall components, and also that the generation of O 1 − may be involved during hypersensitive cell death as a trigger of the sequence of resistance reactions.