Book ChapterDOI
Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal
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This chapter discusses the methods used for the qualitative and quantitative determination of aldehydes in biological systems and focuses on 4-hydroxynonenal and malondialdehyde, which are in many instances the most abundant individual aldehyde resulting from lipid peroxidation.Abstract:
Publisher Summary This chapter discusses the methods used for the qualitative and quantitative determination of aldehydes in biological systems. It focuses on 4-hydroxynonenal (HNE) and malondialdehyde (MDA). 4-Hydroxynonenal is produced as a major product of the peroxidative decomposition of polyunsaturated fatty acids (PUFA) and possesses cytotoxic, hepatotoxic, mutagenic, and genoroxic properties. Increased levels of HNE are found in plasma and various organs under conditions of oxidative stress. In addition to HNE, lipid peroxidation generates many other aldehydes that may also be of toxicological significance. Malondialdehyde is in many instances the most abundant individual aldehyde resulting from lipid peroxidation, and its determination by thiobarbituric acid (TBA) is one of the most common assays in lipid peroxidation studies. In vitro MDA can alter proteins, DNA, RNA, and many other biomolecules. Recently, it has been demonstrated with monoclonal antibodies that malonaldehyde-altered protein occurs in atheroma of hyperlipidemic rabbits.read more
Citations
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Journal ArticleDOI
Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes.
TL;DR: This review provides a comprehensive summary on the chemical properties of 4-hydroxyalkenals and malonaldehyde, the mechanisms of their formation and their occurrence in biological systems and methods for their determination, as well as the many types of biological activities described so far.
Journal ArticleDOI
Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal.
TL;DR: This review focuses on biochemical concepts of lipidPeroxidation, production, metabolism, and signaling mechanisms of two main omega-6 fatty acids lipid peroxidation products: malondialdehyde (MDA) and, in particular, 4-hydroxy-2-nonenal (4-HNE), summarizing not only its physiological and protective function as signaling molecule stimulating gene expression and cell survival, but also its cytotoxic role inhibiting geneexpression and promoting cell death.
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Pin-Ching Maness,Sharon Smolinski,Daniel M. Blake,Zheng Huang,Edward J. Wolfrum,William A. Jacoby +5 more
TL;DR: It is concluded that TiO2 photocatalysis promoted peroxidation of the polyunsaturated phospholipid component of the lipid membrane initially and induced major disorder in the E. coli cell membrane.
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IKKβ Couples Hepatocyte Death to Cytokine-Driven Compensatory Proliferation that Promotes Chemical Hepatocarcinogenesis
TL;DR: IKKbeta orchestrates inflammatory crosstalk between hepatocytes and hematopoietic-derived cells that promotes chemical hepatocarcinogenesis and blocks excessive DEN-induced carcinogenesis in Ikkbeta(Deltahep) mice.
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Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants.
TL;DR: The mechanism of phenoxyl radical prooxidant activity in terms of lifetime prolongation by spin-stabilizing agents is discussed and Phenoxyl radicals can form an antioxidant system equivalent to that of ascorbate in plant cells.
References
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Journal ArticleDOI
Determination of malonaldehyde precursor in tissues by thiobarbituric acid test
Mitsuru Uchiyama,Midori Mihara +1 more
TL;DR: It was concluded that the deproteinization of homogenate prior to coloration is not needed, but double wavelength measurement is necessary to avoid interference and the reaction should be performed with phosphoric acid at a definite pH near 2.0.
Journal ArticleDOI
Autoxidation of human low density lipoprotein: loss of polyunsaturated fatty acids and vitamin E and generation of aldehydes.
TL;DR: The rate and extent of the change of LDL constituents occurring during lipid peroxidation is reported for the first time, and 4-hydroxynonenal was most effective, followed by 2,4-heptadienal, hexanal, and malonaldehyde.
Journal ArticleDOI
Malondialdehyde-altered protein occurs in atheroma of Watanabe heritable hyperlipidemic rabbits
TL;DR: Immuno-cytochemical analyses reveal the presence of protein modified by malondialdehyde, a peroxidative end product, which colocalizes with the extracellular deposition of apolipoprotein B-100 protein of LDL in atheroma from Watanabe heritable hyperlipidemic rabbits, providing direct evidence for the existence in vivo ofprotein modified by a physiological product of lipid peroxidation within arterial lesions.
Journal ArticleDOI
Evidence that Peroxidation of Lysosomal Membranes Is Initiated by Hydroxyl Free Radicals Produced during Flavin Enzyme Activity
TL;DR: It was demonstrated that the superoxide free radical per se was not the agent causing lysis, and evidence is presented that the free radical causing the lysis of the lysosomes is the hydroxyl free radical (OH·).