Diagnostic Potential of Saliva: Current State and Future Applications
Tina Pfaffe,Justin J. Cooper-White,Peter Beyerlein,Karam Kostner,Karam Kostner,Chamindie Punyadeera +5 more
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TLDR
Saliva has the potential to become a first-line diagnostic sample of choice owing to the advancements in detection technologies coupled with combinations of biomolecules with clinical relevance as mentioned in this paper, however, these technologies have not yet been integrated into current clinical practice and work flow.Abstract:
BACKGROUND: Over the past 10 years, the use of saliva as a diagnostic fluid has gained attention and has become a translational research success story. Some of the current nanotechnologies have been demonstrated to have the analytical sensitivity required for the use of saliva as a diagnostic medium to detect and predict disease progression. However, these technologies have not yet been integrated into current clinical practice and work flow. CONTENT: As a diagnostic fluid, saliva offers advantages over serum because it can be collected noninvasively by individuals with modest training, and it offers a cost-effective approach for the screening of large populations. Gland-specific saliva can also be used for diagnosis of pathology specific to one of the major salivary glands. There is minimal risk of contracting infections during saliva collection, and saliva can be used in clinically challenging situations, such as obtaining samples from children or handicapped or anxious patients, in whom blood sampling could be a difficult act to perform. In this review we highlight the production of and secretion of saliva, the salivary proteome, transportation of biomolecules from blood capillaries to salivary glands, and the diagnostic potential of saliva for use in detection of cardiovascular disease and oral and breast cancers. We also highlight the barriers to application of saliva testing and its advancement in clinical settings. SUMMARY: Saliva has the potential to become a first-line diagnostic sample of choice owing to the advancements in detection technologies coupled with combinations of biomolecules with clinical relevance.read more
Citations
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Comparison of Widely Used Biochemical Analytes in the Serum and Saliva Samples of Dialysis Patients.
Medine Alpdemir,Mehmet Akif Eryilmaz,Mehmet Fatih Alpdemir,Güler Topçu,Alper Azak,Doğan Yücel +5 more
TL;DR: Higher salivary urea and creatinine levels in patient groups, consistent with serum levels, may be useful in the evaluation of azotemia in dialysis patients.
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Noninvasive determination of human cortisol and dehydroepiandrosterone sulfate using liquid chromatography-tandem mass spectrometry
TL;DR: An LC-MS/MS method for quantifying cortisol and DHEA-S in human saliva was developed and validated for clinical and psychoneuroendocrinological research that require noninvasive means of measuring these hormones.
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Difficulties detecting miRNA-203 in human whole saliva by the use of PCR.
TL;DR: To make HWS a valuable source for different analyses, more sensitive PCR techniques capable of detecting very low levels of mRNAand miRNA as well as more efficient methods for extraction of RNA are needed.
Journal ArticleDOI
Simultaneous quantification of 18 saturated and unsaturated fatty acids and 7 sterols as their tert-butyldimethylsilyl derivatives in human saliva using gas chromatography-tandem mass spectrometry
Ju-Yeon Moon,Tae Yeon Kong,Hyun-Jun Jang,Han Chang Kang,Yong-Yeon Cho,Joo Young Lee,Hye Suk Lee +6 more
TL;DR: Gas chromatography-tandem mass spectrometry using one-step tert-butyldimethylsilyl (TBDMS) derivatization was developed for comprehensive profiling of 18 FAs and 7 sterols and can be used as a primary screening tool to examine the levels of both FAs or sterols in saliva, providing detailed information about their homeostasis for diagnostic and prognostic purposes.
Journal ArticleDOI
Estimation of Proinflammatory Factors in the Saliva of Adult Patients with Cystic Fibrosis and Dental Caries.
Tomasz Hildebrandt,Anna Zawilska,Agata Trzcionka,Marta Tanasiewicz,Henryk Mazurek,Elżbieta Świętochowska +5 more
TL;DR: New diagnostic methods, including the evaluation of selected salivary biochemical parameters, may indicate the existence of factors predisposing to severe tooth decay in the study group.
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