Differential gene expression induced by Verteporfin in endometrial cancer cells.
TLDR
RNA sequencing data revealed multiple key genes of pathological significance in EMCA, thereby improving the understanding of molecular profiles of EMCA cells and indicating that subcutaneous tumors in mice were regressed after VP treatment by inhibiting cell cycle pathway proteins.Abstract:
Endometrial cancer (EMCA) is a clinically heterogeneous disease. Previously, we tested the efficacy of Verteporfin (VP) in EMCA cells and observed cytotoxic and anti-proliferative effects. In this study, we analyzed RNA sequencing data to investigate the comprehensive transcriptomic landscape of VP treated Type 1 EMCA cell lines, including HEC-1-A and HEC-1-B. There were 549 genes with differential expression of two-fold or greater and P < 0.05 after false discovery rate correction for the HEC-1-B cell line. Positive regulation of TGFβ1 production, regulation of lipoprotein metabolic process, cell adhesion, endodermal cell differentiation, formation and development, and integrin mediated signaling pathway were among the significantly associated terms. A functional enrichment analysis of differentially expressed genes after VP treatment revealed extracellular matrix organization Gene Ontology as the most significant. CDC23 and BUB1B, two genes crucially involved in mitotic checkpoint progression, were found to be the pair with the best association from STRING among differentially expressed genes in VP treated HEC-1-B cells. Our in vivo results indicate that subcutaneous tumors in mice were regressed after VP treatment by inhibiting cell cycle pathway proteins. The present study revealed multiple key genes of pathological significance in EMCA, thereby improving our understanding of molecular profiles of EMCA cells.read more
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YAP/TAZ Transcriptional Coactivators Create Therapeutic Vulnerability to Verteporfin in EGFR-mutant Glioblastoma.
Krishanthan Vigneswaran,Nathaniel H. Boyd,Se-Yeong Oh,Shoeb Lallani,Andrew B. Boucher,Stewart G. Neill,Jeffrey J. Olson,Renee Read +7 more
TL;DR: The benzoporphyrin derivative verteporfin, a disruptor of YAP/TAZ-TEAD–mediated transcription, preferentially induced apoptosis of cultured patient-derived EGFR-amplified/mutant GBM cells, and conferred significant survival benefit in an orthotopic xenograft GBM model.
Journal ArticleDOI
The Role of Photoactivated and Non-Photoactivated Verteporfin on Tumor.
Changran Wei,Xiangqi Li +1 more
TL;DR: Based on the present evidence, VP may be repositioned as a promising anti-cancer chemotherapeutic and adjuvant drug in the absence of light activation against cancer cells and solid tumors.
Journal ArticleDOI
Impact of land-use changes on the genesis and evolution of extreme rainfall event: a case study over Uttarakhand, India
TL;DR: In this paper, the impact assessment of different land-use data sets on the simulation of an Extreme Rainfall Event (ERE) which is one of the unusually rare events that occurred between 14th to 18th of June 2013 over Uttarakhand in India.
Journal Article
Verteporfin induced SUMOylation of YAP1 in endometrial cancer.
TL;DR: This study analyzed the global effects of Verteporfin on cell function by using Reverse Phase Protein Arrays (RPPA) and Ingenuity Pathway Analysis (IPA) and found that the SUMOylation of Y AP1 was regulated by YAP1 phosphorylation.
Journal ArticleDOI
Identification of a five‑gene signature for predicting the progression and prognosis of stage I endometrial carcinoma
TL;DR: A 5-gene signature that can be used to predict the progression of UCEC was revealed and four genes were highly expressed in endometrial cancerous tissues compared with normalendometrial tissues at the protein level.
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