Journal ArticleDOI
Differential label‐free quantitative proteomic analysis of avian eggshell matrix and uterine fluid proteins associated with eggshell mechanical property
Congjiao Sun,Guiyun Xu,Ning Yang +2 more
TLDR
15 proteins mainly relating to eggshell matrix specific proteins, calcium binding and transportation, protein folding and sorting, bone development or diseases, and thyroid hormone activity were considered to have closer association with the formation of strong eggshell.Abstract:
Eggshell strength is a crucial economic trait for table egg production. During the process of eggshell formation, uncalcified eggs are bathed in uterine fluid that plays regulatory roles in eggshell calcification. In this study, a label-free MS-based protein quantification technology was used to detect differences in protein abundance between eggshell matrix from strong and weak eggs (shell matrix protein from strong eggshells and shell matrix protein from weak eggshells) and between the corresponding uterine fluids bathing strong and weak eggs (uterine fluid bathing strong eggs and uterine fluid bathing weak eggs) in a chicken population. Here, we reported the first global proteomic analysis of uterine fluid. A total of 577 and 466 proteins were identified in uterine fluid and eggshell matrix, respectively. Of 447 identified proteins in uterine fluid bathing strong eggs, up to 357 (80%) proteins were in common with proteins in uterine fluid bathing weak eggs. Similarly, up to 83% (328/396) of the proteins in shell matrix protein from strong eggshells were in common with the proteins in shell matrix protein from weak eggshells. The large amount of common proteins indicated that the difference in protein abundance should play essential roles in influencing eggshell strength. Ultimately, 15 proteins mainly relating to eggshell matrix specific proteins, calcium binding and transportation, protein folding and sorting, bone development or diseases, and thyroid hormone activity were considered to have closer association with the formation of strong eggshell.read more
Citations
More filters
Journal ArticleDOI
Quantitative proteomics and bioinformatic analysis provide new insight into protein function during avian eggshell biomineralization
Pauline Marie,Valérie Labas,Aurélien Brionne,Grégoire Harichaux,Christelle Hennequet-Antier,Yves Nys,Joël Gautron +6 more
TL;DR: Qualitative and quantitative proteomics of the uterine fluid constituents, coupled with bioinformatic analysis, are used to predict the functional role of proteins secreted at each of the three main stages of shell calcification to determine the importance of particular proteins relative to eggshell mineralization.
Journal ArticleDOI
Novel identification of matrix proteins involved in calcitic biomineralization.
TL;DR: Cross-analysis of proteomic data from two mineralized models, fertilized and unfertilized chicken eggshells, identified proteins associated with the mammillary cones that are the sites of initiation of eggshell formation and several cone-resident proteins that are candidates to regulate initiation of Eggshell calcification.
Journal Article
Biochemistry of the organic matrix of the eggshell [Collagen].
TL;DR: A detailed discussion of the biochemistry of the first three organic constituents of the eggshell can be found in this article, including shell membranes, mammillary cores, shell matrix, and cuticle.
Journal ArticleDOI
Quantitative proteomics provides new insights into chicken eggshell matrix protein functions during the primary events of mineralisation and the active calcification phase.
Pauline Marie,Valérie Labas,Aurélien Brionne,Grégoire Harichaux,Christelle Hennequet-Antier,Alejandro B. Rodríguez-Navarro,Yves Nys,Joël Gautron +7 more
TL;DR: In this paper, the authors used quantitative proteomics to study four key stages of shell mineralisation: widespread deposition of amorphous calcium carbonate (ACC), transformation into crystalline calcite aggregates, formation of larger calcite crystal units and development of a columnar structure with preferential calcite orientation.
Journal ArticleDOI
In-depth comparative analysis of the chicken eggshell membrane proteome.
TL;DR: The results highlight the structural nature of the ESM constituents that are relevant to various biomedical applications, such as wound healing, and significantly enriched functional groups that reinforce food safety of the table egg.
References
More filters
Journal ArticleDOI
Mass spectrometry-based proteomics
Ruedi Aebersold,Matthias Mann +1 more
TL;DR: The ability of mass spectrometry to identify and, increasingly, to precisely quantify thousands of proteins from complex samples can be expected to impact broadly on biology and medicine.
Journal ArticleDOI
Molecular chaperones in cellular protein folding.
TL;DR: Significant progress has been made in the understanding of the ATP-dependent mechanisms used by the Hsp70 and chaperonin families of molecular chaperones, which can cooperate to assist in folding new polypeptide chains.
Journal ArticleDOI
A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics
TL;DR: A linear dynamic range over 2 orders of magnitude is demonstrated by using the number of spectra (spectral sampling) acquired for each protein by the data-dependent acquisition of peptides eluting into the mass spectrometer.
Journal ArticleDOI
Quantitative mass spectrometry in proteomics: a critical review
TL;DR: This review critically examine the more commonly used quantitative mass spectrometry methods for their individual merits and discusses challenges in arriving at meaningful interpretations of quantitative proteomic data.
Journal ArticleDOI
The Paragon Algorithm, a Next Generation Search Engine That Uses Sequence Temperature Values and Feature Probabilities to Identify Peptides from Tandem Mass Spectra
Ignat V. Shilov,Sean L. Seymour,Alpesh A. Patel,Alex Loboda,Wilfred H. Tang,Sean P. Keating,Christie L. Hunter,Lydia M. Nuwaysir,Daniel A. Schaeffer +8 more
TL;DR: The Paragon™ Algorithm, a novel database search engine for the identification of peptides from tandem mass spectrometry data, is presented and a comparison with Mascot is presented for a series of analogous searches to explore the relative impact of increasing search space.