Differentiation and function of rat adipocyte precursor cells in primary culture.
TLDR
This cell preparation has a potential of yielding information of physiological significance in studies of the regulation of adipocyte multiplication, and shows more metabolic activity in pathways for fatty acid incorporation from exogenous lipid sources (lipoprotein lipase activity) than from de novo synthesis.About:
This article is published in Journal of Lipid Research.The article was published on 1980-08-01 and is currently open access. It has received 143 citations till now. The article focuses on the topics: Adipocyte & Lipoprotein lipase.read more
Citations
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Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium.
Hans Hauner,G Entenmann,Martin Wabitsch,Danielle Gaillard,Gérard Ailhaud,Raymond Negrel,Ernst-Friedrich Pfeiffer +6 more
TL;DR: It is concluded that adult human adipose tissue still contains precursor cells that are able to undergo adipose differentiation in vitro and this improved culture system may offer the opportunity to characterize other adipogenic factors as well as antiadipogenic factors involved in the control of adipose tissues growth.
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Hormonal regulation of hormone-sensitive lipase activity and mRNA levels in isolated rat adipocytes.
TL;DR: Hormone-sensitive lipase (HSL) mediates the lipolysis of triacylglycerol from mammalian adipocytes, resulting in the release of non-esterified fatty acids and glycerol and the measurement of HSL mRNA levels in response to hormonal regulators was studied.
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Primary cultures of unilocular fat cells: Characteristics of growth in vitro and changes in differentiation properties
TL;DR: A new method termed 'ceiling culture' for culturing in vitro unilocular fat cells obtained from humans and rats is described, which can be maintained in culture for long periods of time and continue to exhibit specific functions such as lipogenesis and lipolysis.
Journal ArticleDOI
Mature adipocytes, but not preadipocytes, promote the growth of breast carcinoma cells in collagen gel matrix culture through cancer-stromal cell interactions
TL;DR: The data suggest that mature adipocytes may be involved in the mechanisms regulating the growth of breast cancer mass through their growth‐promoting effect on ER‐positive tumour cells.
Journal ArticleDOI
Requirement and role of arachidonic acid in the differentiation of pre-adipose cells
TL;DR: In this paper, the authors showed that arachidonic acid from intracellular stores and/or of exogenous origin can play a major role for the onset of critical mitoses leading to terminal differentiation in pre-adipose cells.
References
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An established pre-adipose cell line and its differentiation in culture
Howard Green,Mark Meuth +1 more
TL;DR: The conversion of 3T3-L1 from pre-adipose to adipose cells therefore involves a process of differentiation which can be studied under cell culture conditions.
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Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells
Howard Green,Olanlyl Kehinde +1 more
TL;DR: Starting with a clone converting to adipose cells with a vary low frequency, it has been possible, by serial selection, to generate subclones which convert with a high frequency, due to spontaneous heritable changes affecting susceptibility to the adipose conversion.
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Diet-induced adipocyte number increase in adult rats: a new model of obesity.
TL;DR: In this article, an analysis of adipose tissue morphology changes during the course of diet-induced weight gain suggests that the achievement of some specific mean adipocyte size triggers the events that culminate in adipocyte number increase.
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Effect of age, sex, and sites on the cellularity of the adipose tissue in mice and rats rendered obese by a high-fat diet.
TL;DR: The level of fat in a diet is involved in both formation and maturation of new fat cells and in the regulation of fat cell lipid content and the two processes may be separated or may act together according to the adipose tissue site.
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Cytological and enzymological characterization of adult human adipocyte precursors in culture.
R L Van,C E Bayliss,D A Roncari +2 more
TL;DR: Subcellular preparations of the cells grown from the stromal-vascular fraction revealed lipoprotein lipase activity that was not detectable in skin fibroblasts that was significant in each case.