Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism.
Reads0
Chats0
TLDR
The authors investigated whether a default mechanism of neural specification could regulate the acquisition of neural stem cell identity directly from embryonic stem (ES) cells, and found that the default mechanism is negatively regulated by TGFβ-related signaling.About:
This article is published in Neuron.The article was published on 2001-04-01 and is currently open access. It has received 796 citations till now. The article focuses on the topics: Neurosphere & Neural stem cell.read more
Citations
More filters
Journal ArticleDOI
BMP Induction of Id Proteins Suppresses Differentiation and Sustains Embryonic Stem Cell Self-Renewal in Collaboration with STAT3
TL;DR: It is reported that bone morphogenetic proteins (BMPs) act in combination with LIF to sustain self-renewal and preserve multilineage differentiation, chimera colonization, and germline transmission properties.
Journal ArticleDOI
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
TL;DR: The transcriptional profiles of mouse embryonic, neural, and hematopoietic stem cells were compared to define a genetic program for stem cells and provide a foundation for a more detailed understanding of stem cell biology.
Journal ArticleDOI
Directed Differentiation of Embryonic Stem Cells into Motor Neurons
TL;DR: It is shown that developmentally relevant signaling factors can induce mouse embryonic stem cells to differentiate into spinal progenitor cells, and subsequently into motor neurons, through a pathway recapitulating that used in vivo.
Journal ArticleDOI
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.
TL;DR: It is reported that neither multicellular aggregation nor coculture is necessary for ES cells to commit efficiently to a neural fate and this system provides a platform for defining the molecular machinery of neural commitment and optimizing the efficiency of neuronal and glial cell production from pluripotent mammalian stem cells.
Journal ArticleDOI
The development of neural stem cells
TL;DR: A rush of papers proclaiming adult stem cell plasticity has fostered the notion that there is essentially one stem cell type that, with the right impetus, can create whatever progeny the authors' heart, liver or other vital organ desires, but studies aimed at understanding the role of stem cells during development have led to a different view — that stem cells are restricted regionally and temporally, and thus not all stem cell types are equivalent.
References
More filters
Journal ArticleDOI
Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
Journal ArticleDOI
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.
Journal ArticleDOI
The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium
TL;DR: The in vitro developmental potential of mouse blastocyst-derived embryonic stem cell lines and the consistency with which the cells express this potential are presented as aspects which open up new approaches to the investigation of embryogenesis.