Journal ArticleDOI
Dna damage in the kidneys of diabetic rats exhibiting microalbuminuria
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TLDR
Daily injection of insulin starting on the third day after streptozotocin treatment significantly reduced both urinary albumin excretion and papillary 8-OHdG formation, which suggests that these are associated with the diabetic state induced by streptoocin rather than a direct nephrotoxic effect of the drug.About:
This article is published in Free Radical Biology and Medicine.The article was published on 1994-02-01. It has received 116 citations till now. The article focuses on the topics: Streptozotocin & Microalbuminuria.read more
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Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis
TL;DR: The increase of the 8-OH-dG level in the cellular DNA, detected by HPLC-ECD method, was supported by its immunochemical detection and its enhanced repair activity, and the validity of the general use of 8-Hydroxy-2'-deoxyguanosine as a marker of cellular oxidative stress is discussed.
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Nox4 NAD(P)H oxidase mediates hypertrophy and fibronectin expression in the diabetic kidney.
Yves Gorin,Karen Block,James Hernandez,Basant Bhandari,Brent Wagner,Jeffrey L. Barnes,Jeffrey L. Barnes,Hanna E. Abboud,Hanna E. Abboud +8 more
TL;DR: A role for Nox4 is established as the major source of ROS in the kidneys during early stages of diabetes and it is established that Nox 4-derived ROS mediate renal hypertrophy and increased fibronectin expression.
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Protein Kinase C–Dependent Increase in Reactive Oxygen Species (ROS) Production in Vascular Tissues of Diabetes: Role of Vascular NAD(P)H Oxidase
Toyoshi Inoguchi,Toshiyo Sonta,Hirotaka Tsubouchi,Takashi Etoh,Maiko Kakimoto,Noriyuki Sonoda,Naoichi Sato,Naotaka Sekiguchi,Kunihisa Kobayashi,Hideki Sumimoto,Hideo Utsumi,Hajime Nawata +11 more
TL;DR: Findings strongly suggest that the PKC-dependent activation of NAD(P)H oxidase may be an essential mechanism responsible for increased oxidative stress in diabetes.
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Impact of mitochondrial ROS production in the pathogenesis of diabetes mellitus and its complications.
Takeshi Nishikawa,Eiichi Araki +1 more
TL;DR: The results suggest the importance of mitochondrial ROS in the pathogenesis of diabetes mellitus and its complications through modification of various cellular events in many tissues, including vessels, kidney, pancreatic beta cells, and liver.
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NAD(P)H oxidase and uncoupled nitric oxide synthase are major sources of glomerular superoxide in rats with experimental diabetic nephropathy
Minoru Satoh,Sohachi Fujimoto,Yoshisuke Haruna,Sayaka Arakawa,Hideyuki Horike,Norio Komai,Tamaki Sasaki,Katsuhiko Tsujioka,Hirofumi Makino,Naoki Kashihara +9 more
TL;DR: The results indicate that NAD(P)H oxidase and uncoupling of eNOS contribute to glomerular ROS production, mediated by the loss of BH4 availability, and these mechanisms are potential key targets for therapeutic interventions.
References
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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Role of Oxidative Stress in Development of Complications in Diabetes
TL;DR: Structural characterization of the cross-links and other products accumulating in collagen in diabetes is needed to gain a better understanding of the relationship between oxidative stress and the development of complications in diabetes.
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Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury.
TL;DR: The conclusion is reached that MDA determination and the TBA test can offer, at best, a narrow and somewhat empirical window on the complex process of lipid peroxidation.
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The measurement and mechanism of lipid peroxidation in biological systems.
TL;DR: Currently available assays for measuring peroxidation are reviewed--the more specific the assay used, the less peroxide is found in healthy human tissues and body fluids.
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Oxidative damage to DNA during aging: 8-hydroxy-2'-deoxyguanosine in rat organ DNA and urine.
TL;DR: The results suggest that the age-dependent accumulation of oh8dG residues observed in DNA from liver, kidney, and intestine is principally due to the slow loss of DNA nuclease activity; however, an increase in the rate of oxidative DNA damage cannot be ruled out.