Patent
DNA sequence encoding endoglucanase III cellulase
TLDR
In this paper, the EG III cellulase enzyme from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms of the enzyme were presented.Abstract:
The present invention is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of Trichoderma spp.read more
Citations
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Journal ArticleDOI
Microbial cellulases: Engineering, production and applications
Veeresh Juturu,Jin Chuan Wu +1 more
TL;DR: The lack of a better understanding of the mechanisms of individual cellulases, their synergistic actions and their high prices are the major bottlenecks yet to be overcome for large scale commercial applications of cellulases in lignocellulose-based biorefinery.
Patent
Novel Trichoderma Genes
TL;DR: In this article, two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and another encoding an acetylxylanesterase are described.
Patent
Self-processing plants and plant parts
TL;DR: In this paper, synthetic polynucleotides are used to encode mesophilic, thermophilic or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate.
Patent
Trichoderma reesei glucoamylase and homologs thereof
Nigel Dunn-Coleman,Paulien Neefe-Kruithof,Craig E. Pilgrim,Pieter Van Solingen,Donald E. Ward +4 more
TL;DR: In this paper, a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof has been described, which is related to DNA sequences coding for the GluCoAmylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the gluCoamylases.
Patent
BGL7 Beta-Glucosidase and Nucleic Acids Encoding the Same
Nigel Dunn-Coleman,Michael Ward +1 more
TL;DR: In this paper, a β-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence were provided, along with expression vectors and host cells.
References
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Journal ArticleDOI
EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme
Markku Saloheimo,Päivi Lehtovaara,Merja Penttilä,TT Teeri,Jerry Ståhlberg,Gunnar Johansson,Göran Pettersson,Marc Claeyssens,Peter Tomme,J. Knowles +9 more
TL;DR: Comparison of the fungal cellulase structures has led to re-evaluation of hypotheses concerning the localization of the active sites, and all the four T. reesei cellulases share two common, adjacent sequence domains, which apparently can be removed by proteolysis.
Journal ArticleDOI
The cellulase of Trichoderma viride. Purification, characterization and comparison of all detectable endoglucanases, exoglucanases and beta-glucosidases.
TL;DR: It was concluded that these exoglucanases are primarily active on newly generated chain ends and are cellobiohydrolases as judged by high-pressure liquid chromatography of the products obtained from incubation with H3PO4-swollen cellulose.
Journal ArticleDOI
Purification and characterization of a low molecular weight 1,4-beta-glucan glucanohydrolase from the cellulolytic fungus Trichoderma viride QM 9414.
TL;DR: A low molecular weight 1,4-beta-glucan glucanohydrolase (endoglucanase), isolated from culture filtrates of the fungus Trichoderma viride QM 9414 by a two-step procedure of gel filtration and ion-exchange chromatography, was not a carbohydrate-containing protein.
Patent
A cellulase preparation
TL;DR: A cellulase preparation useful for reducing the harshness of cotton-containing fabrics and reducing the rate at which such fabrics become harsh comprises about 40 % or more (based on the total protein content) of an endoglucanase component with a high endoase activity and affinity towards cellulose as mentioned in this paper.
Journal ArticleDOI
Cloning and sequence analysis of a cDNA for cellulase (FI-CMCase) from Aspergillus aculeatus.
Toshihiko Ooi,Atsuhiko Shinmyo,Hirosuke Okada,Saburo Hara,Tokuji Ikenaka,Sawao Murao,Motoo Arai +6 more
TL;DR: This study is the first example of cDNA cloning of an endoglucanase from the genus Aspergillus, and no distinct homology was found between the amino acid sequence of FI-CMCase and known cellulase sequences of other microorganisms.