Double-Bond Requirement in a Fatty Acid Desaturase Mutant of Saccharomyces cerevisiae
Reads0
Chats0
TLDR
It was concluded that the presence of a Delta(9)cis double bond is necessary for growth and the need for a specific chain length or a specific number of double bonds, or for both, is more flexible.Abstract:
A fatty acid Δ9-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Δ9cis double bond is necessary for growth. The need for a specific chain length or a specific number of double bonds, or for both, is more flexible. Tracer-containing 16:1Δ9cis, 18:1Δ9cis, 18:2Δ9,12cis,cis and 18:3Δ9,12,15 all cis revealed that each of these growth-supporting components, once taken up, was not converted into other fatty acids. Concentration effects on doubling time were also considered.read more
Citations
More filters
Journal ArticleDOI
Microbial Fatty Acids and Thermal Adaptation
Merja Suutari,Siino Laakso +1 more
TL;DR: The models presented enable the prediction of temperature-induced changes occurring in the fatty acids of microorganisms, and enzymatic steps of the fatty acid biosynthesis that possibly are under temperature control.
Journal ArticleDOI
Isolation and characterization of OLE1, a gene affecting fatty acid desaturation from Saccharomyces cerevisiae.
TL;DR: The results indicate that 1) the OLE1 gene is essential for production of monounsaturated fatty acids and is probably the structural gene for the delta-9 desaturase enzyme and 2) A large part of membrane ufas present under normal culture conditions are not essential for growth and cell division.
Journal ArticleDOI
Lipids of yeasts.
J B Rattray,A Schibeci,D K Kidby +2 more
TL;DR: A comparison study of the phytochemical properties of anthracene and its application in the context of cradle-to-grave organ transplantation.
Journal ArticleDOI
Nutritional regulation of yeast delta-9 fatty acid desaturase activity.
M A Bossie,C E Martin +1 more
TL;DR: Northern (RNA blot) and slot-blot analyses of mRNA encoded by the OLE1 gene, which appears to be the structural gene for the delta-9 desaturase, indicated that it was sharply reduced in unsaturated-fatty-acid-fed cells, suggesting that a significant part of the regulation involves modulation of available transcripts.
Journal ArticleDOI
Membrane properties of saturated fatty acid mutants of yeast revealed by spin labels.
Susan A. Henry,Alec D. Keith +1 more
TL;DR: It is inferred that the ionic interaction between membrane lipids and membrane proteins is detectable byspin labels and the apparent “fluidity” of membranes detectable by spin labels requires re-evaluation.
References
More filters
Journal ArticleDOI
Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium
A. A. Andreasen,T. J. B. Stier +1 more
Journal ArticleDOI
Influence of trace amounts of fatty acids on the growth of microorganisms.
TL;DR: A better understanding of the inhibitory and and linolenic acids on pneumococci at a concenstimulating effects of fatty acids on the growth of tration may contribute to a clearer bactericidal influence.
Journal ArticleDOI
Biosynthesis of unsaturated fatty acids in microorganisms.
J. Erwin,Konrad Bloch +1 more