Early Events in the Fusarium verticillioides-Maize Interaction Characterized by Using a Green Fluorescent Protein-Expressing Transgenic Isolate
TLDR
The results imply that asymptomatic systemic infection is characterized by a mode of fungal development that includes infection of certain tissues, intercellular growth of a limited number ofFungal hyphae, and reproduction of the fungus in a few cells without invasion of other cells.Abstract:
The infection of maize by Fusarium verticillioides can result in highly variable disease symptoms ranging from asymptomatic plants to severe rotting and wilting. We produced F. verticillioides green fluorescent protein-expressing transgenic isolates and used them to characterize early events in the F. verticillioides-maize interaction that may affect later symptom appearance. Plants grown in F. verticillioides-infested soil were smaller and chlorotic. The fungus colonized all of the underground parts of a plant but was found primarily in lateral roots and mesocotyl tissue. In some mesocotyl cells, conidia were produced within 14 to 21 days after infection. Intercellular mycelium was detected, but additional cells were not infected until 21 days after planting. At 25 to 30 days after planting, the mesocotyl and main roots were heavily infected, and rotting developed in these tissues. Other tissues, including the adventitious roots and the stem, appeared to be healthy and contained only a small number of hyphae. These results imply that asymptomatic systemic infection is characterized by a mode of fungal development that includes infection of certain tissues, intercellular growth of a limited number of fungal hyphae, and reproduction of the fungus in a few cells without invasion of other cells. Development of visibly rotted tissue is associated with massive production of fungal mycelium and much less organized growth.read more
Citations
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Multifaceted Interactions Between Endophytes and Plant: Developments and Prospects
TL;DR: The concept of endophytism is discussed, looking into the latest insights related to the multifarious interactions beneficial for the host plant and exploring the importance of these associations in agriculture and the environment and in other vital aspects such as human health.
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Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level.
Lilia Reneé Cavaglieri,Julieta Orlando,María Inés Rodríguez,Sofia Noemi Chulze,Miriam Etcheverry +4 more
TL;DR: The strain B. subtilis CE1 could be a potential biological control agent against F. verticillioides at the root level and the ability of strains of Bacillus to inhibit fungal growth and fumonisin B(1) accumulation in vitro was evaluated.
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Differential interactions of Verticillium longisporum and V. dahliae with Brassica napus detected with molecular and histological techniques
TL;DR: This study confirms that VD is non-pathogenic on B. napus and demonstrates that non-host resistance against this fungus materializes in restriction of systemic spread rather than inhibition of penetration.
Journal ArticleDOI
In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA
Maria Laine Penha Tinoco,Maria Laine Penha Tinoco,Maria Laine Penha Tinoco,Bárbara B. A. Dias,Rebeca C. D’Allastta,Rebeca C. D’Allastta,João Alencar Pamphile,Francisco J. L. Aragão,Francisco J. L. Aragão,Francisco J. L. Aragão +9 more
TL;DR: This study demonstrates for the first time the in vivo interference phenomenon in the pathogenic fungus Fusarium verticillioides, in which expression of an individual fungal transgene was specifically abolished by inoculating mycelial cells in transgenic tobacco plants engineered to express siRNAs from a dsRNA corresponding to the particular transgenes.
Journal ArticleDOI
Transformation-mediated complementation of a FUM gene cluster deletion in Fusarium verticillioides restores both fumonisin production and pathogenicity on maize seedlings.
Anthony E. Glenn,Nicholas C. Zitomer,Anne Marie Zimeri,Lonnie D. Williams,Ronald T. Riley,Robert H. Proctor +5 more
TL;DR: The data indicate that fumonisin production may have been lost by deletion of the FUM cluster in the banana population of F. verticillioides but that it could be restored by molecular genetic complementation.
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