Effect of Human Testicular Cells Conditioned Medium on In Vitro Maturation and Morphology of Mouse Oocytes.
Maryam Adib,Seyed Morteza Seifati,Mahmood Dehghani Ashkezari,Fatemeh Akyash,Arezoo Khoradmehr,Arezoo Khoradmehr,Behrouz Aflatoonian +6 more
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TLDR
The findings show that TCCM supports oocyte IVM in mice and affect oocyte morphology and hTCCM is an effective medium for GV oocyte growth and maturation compared to the control medium.Abstract:
Background: Testicular cell conditioned medium (TCCM) has been shown to induce female germ cell developmentin vitro from embryonic stem cells (ESCs). Testicular cells (TCs) secrete a variety of growth factors such as growthdifferentiation factor-9 (GDF-9), bone morphogenetic protein 4 (BMP-4), stem cell factor (SCF), leukemia inhibitoryfactor (LIF), and other, that could improve oocyte maturation. Here we have investigated the effect of human TCCM(hTCCM) on in vitro maturation (IVM) and morphology of mouse oocytes.Materials and Methods: In this experimental study, 360 germinal vesicle (GV) oocytes were obtained from NMRImice, aged 4-6 weeks that had received 5 IU pregnant mare's serum gonadotropin (PMSG) 48 hours before. GVoocytes were subjected to IVM. 120 GV oocytes were cultured in each medium; hTCCM as the test group, DMEM+ 20%FBS as the control group and Ham’s F10 + HFF medium as the sham group. The rates of the IVM and perivitellinespace (PVS) changes were recorded at 8, 16 and 24 hours after culture. The metaphase II (MII) oocytes weresubjected for in vitro fertilization (IVF) and the fertilization rate was evaluated after 1, 2, and 3 days.Results: There was a significant difference between the maturation rates in hTCCM (31.67% MII) and the control [0% MII,p 0.05). IVF success rate for MII oocytes obtained from IVM in the hTCCMgroup was 28.94% (n=11). Our data showed that hTCCM is an effective medium for GV oocyte growth and maturationcompared to the control medium.Conclusion: Our findings show that TCCM supports oocyte IVM in mice and affect oocyte morphology.read more
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The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study.
Maryam Adib,Seyed Morteza Seifati,Mahmood Dehghani Ashkezari,Arezoo Khoradmehr,Roshan Rezaee-Ranjbar-Sardari,Somayyeh Sadat Tahajjodi,Behrouz Aflatoonian +6 more
TL;DR: In this paper, the effect of human cumulus cell condition medium (hCCCM) on the IVM of immature mouse oocytes and morphology was investigated, where 240 germinal vesile oocytes were collected from four-six wk-old mice after 48 hr of 5IU pregnant mare serum gonadotropin (PMSG) injection and cultured in hCCCM and DMEM + 20% FBS (control group, n = 120).
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Co‐culturing or conditioned medium of Sertoli cells: Which one supports in vitro maturation of bovine oocytes and developmental competency of resulting embryos?
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In vitro maturation or in vitro fertilization for women with polycystic ovaries? A case–control study of 194 treatment cycles
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