Journal ArticleDOI
Effect of radiation on vascular endothelial growth factor expression in the C2 canine mastocytoma cell line.
Ivana Sekis,Wilhelm Gerner,Michael Willmann,Laura Rebuzzi,Alexander Tichy,Martina Patzl,Johann G. Thalhammer,Armin Saalmüller,Miriam Kleiter +8 more
TLDR
The C2 cell line was radiosensitive, and a fraction of cells died via apoptosis in a dose-dependent manner, and in response to radiation, C2 cells did not upregulate VEGF production or VEGFR.Abstract:
Objective—To establish the radiosensitivity and effect of irradiation on vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) expression in the canine mastocytoma cell line C2. Sample Population—Canine mastocytoma cell line C2. Procedures—C2 cells were irradiated with single doses of 2, 4, 6, and 8 Gy. The 3-(4, 5-di-methyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide assay and proliferation assays with (methyl-hydrogen 3) thymidine were used for radiosensitivity experiments. Expression of VEGFR was determined via flow cytometry and apoptotic rate via annexin assay. Human and canine VEGF ELISA kits were evaluated in crossover assay experiments, and the canine kit was used thereafter. Results—C2 cells secreted VEGF constitutively. Radiation did not induce a significant increase in VEGF secretion, regardless of radiation dose. Consistently, radiation did not up-regulate VEGFR. Cell survival rates decreased in a dose-dependent manner. The apoptotic cell fraction had a dose-dependent incre...read more
Citations
More filters
Journal ArticleDOI
Effects of epidermal growth factor receptor kinase inhibition on radiation response in canine osteosarcoma cells.
TL;DR: Erlotinib treatment promoted modest enhancement of radiation effects in canine osteosarcoma cells, and possessed activity as a single agent in some cell lines, indicating a potential role for EGFR inhibition in the treatment of a subset of osteosARcoma patients.
Journal ArticleDOI
Vascular endothelial growth factor in diabetic and nondiabetic canine cataract patients.
TL;DR: Aqueous humor values of VEGF in diabetic dogs are not greater than nondiabetics and may serve to protect the dog against development of diabetic retinopathy.
Journal ArticleDOI
Clinical trial of thalidomide combined with radiotherapy in patients with esophageal cancer
TL;DR: Clinical trial of thalidomide combined with radiotherapy in patients with esophageal cancer was conducted in this article, and the results showed that the combination improved the survival of the patients.
Journal ArticleDOI
Pilot assessment of vascular endothelial growth factor receptors and trafficking pathways in recurrent and metastatic canine subcutaneous mast cell tumours
Lucas Da Silva,Carlos Eduardo Fonseca-Alves,Jennifer J. Thompson,Robert A. Foster,Geoffrey A. Wood,Renée Laufer Amorim,Brenda L. Coomber +6 more
TL;DR: A pilot study demonstrates the potential utility of these markers in a subset of scMCT in dogs by quantifying their association with disease outcome and finding significant positive correlations between VEGFR2 and pVEGFR2, and between c‐CBL and pEGFFR2 levels.
Dissertation
Canine Mast Cell Tumours: Characterization of Subcutaneous Tumours and Receptor Tyrosine Kinase Profiling
TL;DR: The results showed that canine MCT have unique expression profiles and activity of KIT, VEGFR2 and PDGFR, the first that identify autocrine VEGF signalling as a possible survival mechanism for canine M CT.
References
More filters
Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Book
Tumors in Domestic Animals
TL;DR: An overview of cancer pathogenesis, diagnosis, and management can be found in this paper, where the authors present an overview of the main types of cancer, including skin and soft tissues tumours of the human body, hemolymphatic system tumours, joints, muscle, alimentary tract, liver, and gall bladder.
Journal Article
Blockade of the Vascular Endothelial Growth Factor Stress Response Increases the Antitumor Effects of Ionizing Radiation
David H. Gorski,Michael A. Beckett,Nora Jaskowiak,Douglas P. Calvin,Helena J. Mauceri,Rabih M. Salloum,Saraswathy Seetharam,Ann Koons,Danielle M. Hari,Donald Kufe,Ralph R. Weichselbaum +10 more
TL;DR: It is reported that VEGF expression is induced in Lewis lung carcinomas (LLCs) both in vitro and in vivo after exposure to ionizing radiation (IR) and in human tumor cell lines (Seg-1 esophageal adenocarcinoma, SQ20B squamous cell carcinoma, T98 and U87 glioblastomas, and U1 melanoma) in vitro.
Journal ArticleDOI
VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism
Hans-Peter Gerber,Ajay K. Malik,Gregg P. Solar,Daniel Eric Sherman,Xiao Huan Liang,Gloria Meng,Kyu Hong,James C. Marsters,Napoleone Ferrara +8 more
TL;DR: A regulatory loop by which VEGF controls survival of haematopoietic stem cells (HSCs) is described, supporting the involvement in HSC survival of a V EGF-dependent internal autocrine loop mechanism that is resistant to inhibitors that fail to penetrate the intracellular compartment.
Journal ArticleDOI
Role of vascular endothelial growth factor in physiologic and pathologic angiogenesis: Therapeutic implications
TL;DR: Clinical efficacy of antiangiogenic therapy with bevacizumab is being evaluated in several phase 3 trials in various types of cancer, as well as in patients with age-related macular degeneration.