Journal ArticleDOI
Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae.
Jane Mellor,Melanie J. Dobson,N.A. Roberts,Mick F. Tuite,J.S. Emtage,S. White,P.A. Lowe,T. Patel,Alan J. Kingsman,Susan M. Kingsman +9 more
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TLDR
A high-efficiency expression vector is constructed to direct the synthesis of heterologous polypeptides in yeast to produce derivatives of the calf chymosin cDNA gene, which can be readily activated to produce an enzyme which has milk-clotting activity.About:
This article is published in Gene.The article was published on 1983-09-01. It has received 334 citations till now. The article focuses on the topics: Expression vector & Chymosin.read more
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Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae
TL;DR: Stable and unstable mRNAs do not differ significantly in their poly(A) metabolism; deadenylation does not destabilize stable m RNAs; there is no correlation between mRNA decay rate and mRNA size; and the degradation of both stable and unstablemRNAs depends on concomitant translational elongation.
Journal ArticleDOI
Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase
TL;DR: The results indicate that the transaldolase level in S. cerevisiae is insufficient for the efficient utilization of pentose phosphate pathway metabolites.
Journal ArticleDOI
Drosophila Brain Tumor is a translational repressor.
TL;DR: The results of these experiments suggest that NHL domain proteins are recruited to various mRNAs by combinatorial protein-protein interactions.
Journal ArticleDOI
Potential Impact of Increased Use of Biocides in Consumer Products on Prevalence of Antibiotic Resistance
Peter Gilbert,Andrew J. McBain +1 more
TL;DR: It is concluded from this review of the literature that the incorporation of antibacterial agents into a widening sphere of personal products has had little or no impact on the patterns of microbial susceptibility observed in the environment, and the associated risks remain finite.
Journal ArticleDOI
Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin.
J.A. van den Berg,K.J. van der Laken,A.J.J. van Ooyen,T.C.H.M. Renniers,Krijn Rietveld,A. Schaap,Anthony J Brake,R.J. Bishop,K. Schultz,D. Moyer +9 more
TL;DR: It is concluded that the efficient synthetic and secretory capacity of this heterologous protein is a property of the yeast Kluyveromyces, and led to the development of a large scale production process for chymosin.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
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A rapid boiling method for the preparation of bacterial plasmids
David S. Holmes,Michael Quigley +1 more
TL;DR: A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed and can be readily adapted for the preparation of plasmid from liter cultures with quantitative yields.