Journal ArticleDOI
Electrical biosensors and the label free detection of protein disease biomarkers
Xiliang Luo,Jason J. Davis +1 more
Reads0
Chats0
TLDR
This review focuses on recent important advances in label free assays of protein using a number of electrical methods, including those based on electrochemical impedance spectroscopy (EIS), amperometry/voltammetry, potentiometry, conductometry and field-effect methods.Abstract:
Electrical detection methodologies are likely to underpin the progressive drive towards miniaturised, sensitive and portable biomarker detection protocols. In being easily integrated within standard electronic microfabrication formats, and developing capability in microfluidics, the facile multiplexed detection of a range of proteins in a small analytical volume becomes entirely feasible with something costing just a few thousand pounds and benchtop or handheld in scale. In this review, we focus on recent important advances in label free assays of protein using a number of electrical methods, including those based on electrochemical impedance spectroscopy (EIS), amperometry/voltammetry, potentiometry, conductometry and field-effect methods. We introduce their mechanistic features and examples of application and sensitivity. The current state of the art, real world applications and challenges are outlined.read more
Citations
More filters
Journal ArticleDOI
Cancer biomarker detection: recent achievements and challenges
Li-Li Wu,Xiaogang Qu +1 more
TL;DR: An overview of recent advances in cancer biomarker detection is provided and several representative examples using different approaches for each biomarker demonstrate that the multidisciplinary technology-based cancer diagnostics are becoming an increasingly relevant alternative to traditional techniques.
Journal ArticleDOI
Optical Biosensors for the Detection of Pathogenic Microorganisms
Seung Min Yoo,Sang Yup Lee +1 more
TL;DR: The technological and methodological approaches underlying diverse optical-sensing platforms and methods for detecting pathogenic microorganisms are reviewed, together with the strengths and drawbacks of each technique.
Journal ArticleDOI
Advances and challenges in biosensor-based diagnosis of infectious diseases
Mandy L. Y. Sin,Kathleen E. Mach,Kathleen E. Mach,Pak Kin Wong,Joseph C. Liao,Joseph C. Liao +5 more
TL;DR: The advances of biosensor technologies for infectious disease diagnostics are reviewed and the critical challenges that need to be overcome are discussed in order to implement integrated diagnostic biosensors in real world settings.
Journal ArticleDOI
SERS Encoded Silver Pyramids for Attomolar Detection of Multiplexed Disease Biomarkers
Liguang Xu,Wenjing Yan,Wei Ma,Hua Kuang,Wu Xiaoling,Liqaing Liu,Yuan Zhao,Libing Wang,Chuanlai Xu +8 more
TL;DR: Three disease biomarkers can simultaneously be detected at the attomolar level because of a novel surface-enhanced Raman scattering (SERS) encoded silver pyramid sensing system that holds promising potential for biodetection applications.
Journal ArticleDOI
Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics
TL;DR: In this review, it is shown that in the recent years a significant progress was done in the EC analysis of practically all proteins, based on electroactivity of amino acid (aa) residues in proteins.
References
More filters
Journal ArticleDOI
Label-Free Impedance Biosensors: Opportunities and Challenges.
TL;DR: The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout.
Journal ArticleDOI
Controlled Growth and Structures of Molecular-Scale Silicon Nanowires
TL;DR: In this article, single-crystal silicon nanowires with diameters approaching molecular dimensions were synthesized using gold nanocluster-catalyzed 1D growth using high-resolution transmission electron microscopy studies.
Journal ArticleDOI
Analytical applications of aptamers.
TL;DR: Aptamers are suitable for applications based on molecular recognition as analytical, diagnostic and therapeutic tools and can be considered as a valid alternative to antibodies or other bio-mimetic receptors, for the development of biosensors and other analytical methods.
Journal ArticleDOI
The cancer biomarker problem
TL;DR: In this article, the authors identify the biological indicators, or biomarkers, of cancer that predict who will benefit from a particular targeted therapy, which can guide the design of drugs targeted to a relevant molecule.
Journal ArticleDOI
Immobilization strategies to develop enzymatic biosensors
TL;DR: The choice of the immobilization method is shown to represent an important parameter that affects biosensor performances, mainly in terms of sensitivity, selectivity and stability, by influencing enzyme orientation, loading, mobility, stability, structure and biological activity.