Journal ArticleDOI
Enhanced Transfection Efficiency into Macrophages and Dendritic Cells by a Combination Method Using Mannosylated Lipoplexes and Bubble Liposomes with Ultrasound Exposure
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TLDR
It is demonstrated that the gene transfection efficiency in macrophages and dendritic cells was significantly increased by this combination method using mannosylated lipoplexes and BLs with US exposure.Abstract:
To achieve effective gene therapy, it is necessary to selectively and efficiently transfect therapeutic gene into targeted cells. In this study, we developed a combination method using mannosylated lipoplexes, which show selectivity to antigen-presenting cells such as macrophages and dendritic cells, and bubble liposomes (BLs), which are known to enhance transfection efficiency on exposure to ultrasound (US). In cultured mouse macrophages, known for the expression of mannose receptors, the transfection efficiency of this combination method using mannosylated lipoplexes and BLs with US was higher than that of naked pDNA or unmodified lipoplexes and BLs. In the liver and spleen, the in vivo transfection efficiency of this combination method was significantly higher than that of naked pDNA or unmodified lipoplexes and BLs with US. Transfection in hepatic nonparenchymal cells using this combination method was about 12 times higher than that in hepatic parenchymal cells. As far as splenic transfection...read more
Citations
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Liposomal drug delivery systems: from concept to clinical applications.
TL;DR: Lipidic nanoparticles are the first nanomedicine delivery system to make the transition from concept to clinical application, and they are now an established technology platform with considerable clinical acceptance.
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Targeted Liposomal Drug Delivery to Monocytes and Macrophages
TL;DR: The goal for targeting monocytes/macrophages using liposomes includes not only drug delivery but also potentially a role in cell ablation and cell activation for the treatment of conditions including cancer, atherosclerosis, HIV, and chronic inflammation.
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A review of glycosylated carriers for drug delivery.
TL;DR: This review presents an overview of different applications of glycosylated carriers, with a focus on their applicability in development of a nanoconstruct with GRAS status.
Journal ArticleDOI
Cancer gene therapy by IL-12 gene delivery using liposomal bubbles and tumoral ultrasound exposure
Ryo Suzuki,Eisuke Namai,Yusuke Oda,Norihito Nishiie,Shota Otake,Risa Koshima,Keiichi Hirata,Yuichiro Taira,Naoki Utoguchi,Yoichi Negishi,Shinsaku Nakagawa,Kazuo Maruyama +11 more
TL;DR: The combination of Bubble liposomes and ultrasound is a good non-viral vector system in IL-12 cancer gene therapy, and migration of CD8(+) T cells was observed in the mice, indicating that the combination ofbubble liposome collapse (cavitation) is induced by ultrasound exposure.
Journal ArticleDOI
Progress in the development of ultrasound-mediated gene delivery systems utilizing nano- and microbubbles
TL;DR: In this gene delivery system, microstreams and microjets are used as the driving force to transfer genes into cells by opening transient pores in the cell membrane, which can directly deliver plasmid DNA and siRNA into cytosol without endocytosis pathway.
References
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Journal ArticleDOI
Gene therapy progress and prospects: nonviral vectors.
Takuro Niidome,Leaf Huang +1 more
TL;DR: Improvement of delivery methods together with intelligent design of the DNA itself has brought about large degrees of enhancement in the efficiency, specificity and temporal control of nonviral vectors.
Journal ArticleDOI
Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo.
Franz Scherer,Martina Anton,Ulrike Schillinger,Julia Henke,C Bergemann,Achim Krüger,Bernd Gansbacher,Christian Plank +7 more
TL;DR: In this paper, the authors associated gene vectors with superparamagnetic nanoparticles and targeted gene delivery by application of a magnetic field, which potentiated the efficacy of any vector up to several hundred-fold, allowed reduction of the duration of gene delivery to minutes, extended the host tropism of adenoviral vectors to nonpermissive cells and compensated for low retroviral titer.
Journal ArticleDOI
Cancer Immunotherapy by Dendritic Cells
TL;DR: The considerations for generating promising therapeutic antitumor vaccines that use DCs are discussed, and chemotherapies, causing DC activation, enhanced crosspresentation, lymphodepletion, and reduction of immunosuppressive leukocytes, act synergistically with vaccines or adoptive T cell transfer.
Journal Article
Liposome-encapsulated Doxorubicin Targeted to CD44: A Strategy to Kill CD44-overexpressing Tumor Cells
Rom E. Eliaz,Francis C. Szoka +1 more
TL;DR: In both protocols, delivered DOX was significantly more potent than the nonencapsulated DOX in cells expressing high levels of CD44, which suggests that HALs may be a useful targeted drug carrier to treat CD44-expressing tumors.
Journal ArticleDOI
Alternative versus classical activation of macrophages.
Sergij Goerdt,Oliver Politz,Kai Schledzewski,Robert Birk,Alexej Gratchev,Pierre Guillot,Nahid Hakiy,Claus-Detlev Klemke,Edgar Dippel,Vitam Kodelja,Constantin E. Orfanos +10 more
TL;DR: Classically and alternatively activated APC secure the balance between proinflammatory and anti-inflammatory immune reactions.