Open AccessJournal Article
Enhancement of antibody detection in cancer using panel of recombinant tumor-associated antigens.
Jianying Zhang,Carlos A. Casiano,Xuan Xian Peng,James A. Koziol,Edward K. L. Chan,Eng M. Tan +5 more
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TLDR
Breast, lung, and prostate cancer patients showed separate and distinct profiles of reactivity, suggesting that uniquely constituted antigen mini-arrays might be developed to distinguish between some types of cancer.Abstract:
Cancer sera contain antibodies which react with a unique group of autologous cellular antigens called tumor-associated antigens (TAAs) This study determines whether a mini-array of multiple TAAs would enhance antibody detection and be a useful approach to cancer detection and diagnosis The mini-array of TAAs comprised full-length recombinant proteins expressed from cDNAs encoding c-myc, p53, cyclin B1, p62, Koc, IMP1, and survivin Enzyme immunoassay was used to detect antibodies in 527 sera from six different types of cancer Antibody frequency to any individual TAA was variable but rarely exceeded 15-20% With the successive addition of TAAs to a final total of seven antigens, there was a stepwise increase of positive antibody reactions up to a range of 44-68% Breast, lung, and prostate cancer patients showed separate and distinct profiles of reactivity, suggesting that uniquely constituted antigen mini-arrays might be developed to distinguish between some types of cancer Distinct antibody profiles were not observed in gastric, colorectal, and hepatocellular carcinomas with this set of seven TAAs Detection of autoantibodies in cancer can be enhanced by using a mini-array of several TAAs as target antigens Additional studies in early cancer patients and high-risk individuals and the design of unique antigen panels for different cancers would help to determine whether multiple antigen mini-arrays for the detection of autoantibodies might contribute a clinically useful noninvasive approach to cancer detection and diagnosisread more
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Journal ArticleDOI
Survivin, versatile modulation of cell division and apoptosis in cancer
TL;DR: Current evidence and emerging concepts on the multifaceted functions of survivin in cell death and cell division are lined up to line up with how this pathway is being pursued for novel cancer therapeutic strategies.
Journal ArticleDOI
The Sentinel Within: Exploiting the Immune System for Cancer Biomarkers†
Karen S. Anderson,Joshua LaBaer +1 more
TL;DR: Recent advances in proteomics are opening the door for targeted vaccine development, and for using immune response signatures as biomarkers for cancer diagnosis and monitoring.
Journal ArticleDOI
Serum autoantibodies as biomarkers for early cancer detection.
TL;DR: The discovery of panels of tumor‐associated antigens and autoantibody signatures with high sensitivity and specificity would aid in the development of diagnostics, prognostics and therapeutics for cancer patients.
Journal ArticleDOI
A systematic review of humoral immune responses against tumor antigens
TL;DR: Although data on prediagnostic antibody levels are scarce and antibody frequencies for most TAAs are at levels precluding use in diagnostic assays for cancer early detection, there is some promising data on achieving higher sensitivity for cancer detection using panels of TAAs.
Journal ArticleDOI
Autoantibodies in breast cancer: their use as an aid to early diagnosis.
Caroline J. Chapman,Andrea Murray,J Chakrabarti,Alison Thorpe,Caroline M. Woolston,Ugur Sahin,A. Barnes,John F.R. Robertson,John F.R. Robertson +8 more
TL;DR: Autoantibody assays against a panel of antigens could be used as an aid to mammography in the detection and diagnosis of early primary breast cancer, especially in younger women at increased risk of breast cancer where mammography is known to have reduced sensitivity and specificity.
References
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Journal ArticleDOI
A novel anti-apoptosis gene, survivin , expressed in cancer and lymphoma
TL;DR: It is suggested that apoptosis inhibition may be a general feature of neoplasia and survivin is identified as a potential new target for apoptosis-based therapy in cancer and lymphoma.
Book ChapterDOI
Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology
TL;DR: One of the purposes of this chapter is to show that the new molecular biology of cellular antigens and auto-antibodies could now be providing insights into comprehending some features of autoimmunity.
Journal ArticleDOI
Human tumor antigens recognized by t lymphocytes
Thierry Boon,P. van der Bruggen +1 more
TL;DR: It appears increasingly unlikely that immunization of patients against one of these antigens will cause harmful immunological side effects caused by the expression of the relevant gene in the testis, and these conclusions are further strengthened by immunization studies carried out with mouse tumor antigen P815A, which is encoded by a gene that is also expressed only in thetestis.
Journal ArticleDOI
A Survey of the Humoral Immune Response of Cancer Patients to a Panel of Human Tumor Antigens
Elisabeth Stockert,Elke Jager,Yao-Tseng Chen,Matthew J. Scanlan,Ivan Gout,Julia Karbach,Michael Arand,Alexander Knuth,Lloyd J. Old +8 more
TL;DR: To establish a screening system for the humoral response to autoimmunogenic tumor antigens, an enzyme-linked immunosorbent assay (ELISA) was developed using recombinant NY-ESO-1, MAGE-3, SSX2, Melan-A, and tyrosinase proteins.
Journal ArticleDOI
Autoantigen microarrays for multiplex characterization of autoantibody responses
William H. Robinson,Carla DiGennaro,Wolfgang Hueber,Brian B. Haab,Makoto Kamachi,Erik J. Dean,Sylvie Fournel,Derek A. Fong,Mark C. Genovese,Henry E. Neuman de Vegvar,Karl Skriner,David L. Hirschberg,Robert I. Morris,Sylviane Muller,Ger J. M. Pruijn,Walther J. van Venrooij,Josef S. Smolen,Patrick O. Brown,Lawrence Steinman,Paul J. Utz +19 more
TL;DR: This work describes and characterize arrays containing the major autoantigens in eight distinct human autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis, which represents the first report of application of autoantigen microarrays to multiple human disease sera.