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Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology
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The article was published on 1987-06-01 and is currently open access. It has received 4940 citations till now. The article focuses on the topics: Salmonella & Escherichia coli.read more
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
Protein production by auto-induction in high-density shaking cultures
TL;DR: Investigation of factors that affect stability, growth, and induction of T7 expression strains in shaking vessels led to the recognition that sporadic, unintended induction of expression in complex media, previously reported by others, is almost certainly caused by small amounts of lactose.
Patent
Methods for producing members of specific binding pairs
John Mccafferty,Anthony Richard Pope,Kevin Stuart Johnson,Hoogenboom Hendricus R J M,Andrew D. Griffiths,Ronald Henry Jackson,Kaspar Philipp Holliger,James D. Marks,Timothy Piers Clackson,David John Chiswell,Gregory Paul Winter,Timothy Peter Seattle Bonnert +11 more
TL;DR: In this paper, a member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbps members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof.
Journal ArticleDOI
Rapid and simple determination of the Escherichia coli phylogenetic group.
TL;DR: In this paper, a simple and rapid phylogenetic grouping technique based on triplex PCR was proposed, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.
Journal ArticleDOI
Mechanisms of membrane toxicity of hydrocarbons.
TL;DR: In this paper, the authors present general ideas derived from the various reports mentioning toxic effects of lipophilic compounds on the membrane lipid bilayer, affecting the structural and functional properties of these membranes.