Journal ArticleDOI
Evidence for an abundant 33,000-dalton polypeptide regulated by cytokinins in cultured tobacco tissues.
TLDR
The hypothesis that cytokinin in the presence of auxin regulates the production of a specific, major polypeptide in the soluble-protein fraction of the tissue and that this protein is not produced in tissues autotrophic for both auxin and cytokinIn is supported.Abstract:
When cloned pith and leaf tissues of Nicotiana tabacum L. cv. Havana 425 are subcultured for 3 d on auxin-containing medium and labelled for 18 h with [35S]methionine, up to 10% of the labelled, soluble-protein fraction is found in a single band with an apparent molecular weight of approx. 32,000–34,000 dalton on sodium-dodecylsulfate polyacrylamide-gel electrophoretograms. The labelling of this band, designated P33, is dramatically inhibited by the cytokinin, kinetin, in some cell lines at concentrations as low as 1.4·10-8 M. P33 is a major component of the protein fraction obtained from non-habituated clones, cytokinin-habituated clones, and revertant subclones of crown-gall-transformed clones, but cannot be detected in clones habituated for both auxin and cytokinin, or crown-gall-transformed clones. The evidence supports the hypothesis that cytokinin in the presence of auxin regulates the production of a specific, major polypeptide in the soluble-protein fraction of the tissue and that this protein is not produced in tissues autotrophic for both auxin and cytokinin.read more
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A Handbook of Plant Tissue Culture
TL;DR: A Handbook of Plant Tissue Culture by Dr. Philip R. White as discussed by the authors presents the case of the plant biologist, based on 457 references, with commendable force, compressing into this relatively small volume the 'high spots' of plant tissue culture up to the present, in which he has himself played an outstanding part, and points out wide fields of interest for future research.
Journal ArticleDOI
The Physiology and Molecular Biology of Plant 1,3-β-D-Glucanases and 1,3;1,4-β-D-Glucanases
TL;DR: These β-glucanases are structurally related enzymes that are believed to be involved in many important aspects of plant physiology and development, such as germination, growth, defense against pathogens, flowering, cellular and tissue development and differentiation, and probably other roles.
Journal ArticleDOI
Cytokinins as regulators of gene expression
TL;DR: The isolated genes present valuable tools to dissect cytokinin signal transduction pathways and to understand the functional mechanism of this hormone, including its role in cell division, photosynthesis, chloroplast development, disease resistance and nutrient metabolism.
Journal ArticleDOI
Regulation by Cytokinins of Endogenous Levels of Jasmonic and Salicylic Acids in Mechanically Wounded Tobacco Plants
TL;DR: Because transgenic plants constitutively produce four- to six-fold higher amounts of endogenous cytokinin than wild-type plants, it is concluded that cytokinins are indispensable for control of endogenous levels of SA and JA.
Journal ArticleDOI
Developmental and hormonal regulation of β-1,3-glucanase in tobacco.
TL;DR: In this article, a highly sensitive and specific "rocket" immunoassay was used to measure the content of an endo-type β-1,3-glucanase (EC 3.39) in tissues of Nicotiana tabacum L. cv.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
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A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.