Journal ArticleDOI
Evolutionary conservation and versatility of a new set of primers for amplifying the ribosomal internal transcribed spacer regions in insects and other invertebrates
Ya-Jie Ji,De-Xing Zhang,Lijun He +2 more
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TLDR
The availability of these primers to the insect research community should facilitate the use of internal transcribed spacer regions in intraspecific studies as well as phylogenetic analysis of closely related taxa.Abstract:
The evolutionary conservation and versatility of a new set of nuclear primers for the amplification of the ribosomal internal transcribed spacer regions in insects and other invertebrates have been studied. These primers, conserved across Insecta and other invertebrates, are based on a comprehensive taxonomic survey of the current DNA sequence databases. Their versatility was demonstrated by extensive polymerase chain reaction assays in 16 species from two arachnid orders, eight insect orders, three invertebrate and vertebrate chordate orders and by direct sequencing of the amplified products. The availability of these primers to the insect research community should facilitate the use of internal transcribed spacer regions in intraspecific studies as well as phylogenetic analysis of closely related taxa.read more
Citations
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DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae)
TL;DR: If general, these results will increase estimates of global species richness and imply that tropical conservation and host-parasite interactions may be more complex than expected.
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An integrative method for delimiting cohesion species: finding the population-species interface in a group of Californian trapdoor spiders with extreme genetic divergence and geographic structuring.
Jason E. Bond,Amy K. Stockman +1 more
TL;DR: This work presents an objective, repeatable approach to delineating species when populations are divergent and highly structured geographically using the Californian trapdoor spider species complex Aptostichus atomarius Simon as a model system.
Journal ArticleDOI
Strong genetic structure corresponds to small-scale geographic breaks in the Australian alpine grasshopper Kosciuscola tristis
Rachel A. Slatyer,Michael A. Nash,Michael A. Nash,Adam D. Miller,Yoshinori Endo,Yoshinori Endo,Kate D. L. Umbers,Kate D. L. Umbers,Ary A. Hoffmann +8 more
TL;DR: Testing the hypothesis that genetic partitioning reflects observable geographic structuring across Australia’s mainland high country, in the widespread alpine grasshopper Kosciuscola tristis found broadly congruent patterns of regional structure between the DNA sequence and microsatellite datasets corresponding to strong divergence among isolated mountain regions.
Journal ArticleDOI
A Revision of Malagasy Species of Anochetus Mayr and Odontomachus Latreille (Hymenoptera: Formicidae)
Brian L. Fisher,M. Alex Smith +1 more
TL;DR: This study provides an example of collaborative taxonomy, where morphology is combined with DNA barcoding, that allows formalized alpha-taxonomy at a speed, detail, precision, and scale unattainable by employing morphology alone.
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Empirical Assessment of RAD Sequencing for Interspecific Phylogeny
Astrid Cruaud,Mathieu Gautier,Maxime Galan,Julien Foucaud,Laure Sauné,Gwenaëlle Genson,Emeric Dubois,Sabine Nidelet,Thierry Deuve,Jean-Yves Rasplus +9 more
TL;DR: Analysis of the RAD library produced 400× more sites than the Sanger approach, suggesting that RAD-seq is promising to infer phylogeny of eukaryotic species, though potential biases need to be evaluated and new methodologies developed to take full advantage of such data.
References
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Clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.
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'Touchdown' PCR to circumvent spurious priming during gene amplification
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Ribosomal DNA: molecular evolution and phylogenetic inference.
David M. Hillis,Michael T. Dixon +1 more
TL;DR: An analysis of aligned sequences of the four nuclear and two mitochondrial rRNA genes identified regions of these genes that are likely to be useful to address phylogenetic problems over a wide range of levels of divergence.